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10.1111/j.1349-7006.1992.tb00110.x

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suck abstract from ncbi


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pmid1324234
      Jpn+J+Cancer+Res 1992 ; 83 (4 ): 329-33
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  • Direct detection of Epstein-Barr virus DNA from a single Reed-Sternberg cell of Hodgkin s disease by polymerase chain reaction #MMPMID1324234
  • Teramoto N ; Akagi T ; Yoshino T ; Takahashi K ; Jeon HJ
  • Jpn J Cancer Res 1992[Apr]; 83 (4 ): 329-33 PMID1324234 show ga
  • Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein-Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin-embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed-Sternberg cells from all the EBV-positive cases, but not from any of the EBV-negative cases. Background lymphocytes and lysozyme-positive histiocytes from EBV-positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.
  • |Adult [MESH]
  • |Aged [MESH]
  • |Base Sequence [MESH]
  • |DNA, Viral/genetics/*isolation & purification [MESH]
  • |Female [MESH]
  • |Genome, Viral [MESH]
  • |Herpesvirus 4, Human/genetics/*isolation & purification [MESH]
  • |Hodgkin Disease/*microbiology/pathology [MESH]
  • |Humans [MESH]
  • |Male [MESH]
  • |Middle Aged [MESH]
  • |Molecular Sequence Data [MESH]
  • |Oligodeoxyribonucleotides [MESH]
  • |Polymerase Chain Reaction/methods [MESH]
  • |Reed-Sternberg Cells/*microbiology/pathology [MESH]


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