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1991 ; 82
(2
): 206-12
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Release of esterase from murine lymphokine-activated killer cells in
antibody-dependent cellular cytotoxic reaction
#MMPMID1900824
Kato K
; Agatsuma T
; Tanabe T
; Masuko T
; Hashimoto Y
Jpn J Cancer Res
1991[Feb]; 82
(2
): 206-12
PMID1900824
show ga
Release of granule enzyme(s) (BLT esterase) in the antibody dependent
lymphokine-activated killer (LAK) cell-mediated cytotoxic reaction (LAK ADCC) was
studied using LAK cells induced from murine splenocytes and thymocytes, various
human tumor cells and relevant monoclonal antibodies (mAbs) to the tumor cells.
BLT esterase was not significantly released from LAK cells in direct LAK
cell-mediated cytotoxic reactions (LAK CMC). However, cultures of LAK cells and
IgG-coated target tumor cells resulted in release of the enzyme concomitantly
with target cell lysis, although esterase release proceeded faster than target
cell lysis. Anti-LFA-1 mAb showed an inhibitory effect on LAK CMC but not on
either LAK ADCC or BLT esterase release in the ADCC. These results indicate that
exocytosis of granule enzyme from LAK cells is triggered by stimulation of Fc
receptor on LAK cells and that LAK CMC and LAK ADCC differ in their lytic
mechanism in terms of the release of BLT esterase.