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10.1111/j.1349-7006.1989.tb01694.x http://scihub22266oqcxt.onion/10.1111/j.1349-7006.1989.tb01694.x C5917807!5917807!2507489     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Jpn+J+Cancer+Res 1989 ; 80 (7): 662-9 Nephropedia Template TP {{tp|p=2507489|t=1989. Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte?Macrophage Colony?stimulating Factor by Human Lymphokine?activated Killer Cells |pdf=|usr=}}{{2507489}} gab.com Text Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte Macrophage Colony stimulating Factor by Human Lymphokine activated Killer Cells JO: Jpn J Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=2507489 #FOAvip The susceptibilities of human blood monocytes and alveolar macrophages (AM) to cytotoxicity mediated by lymphokine (IL?l)?activated killer (LAK) cells were examined. Monocytes and AM of healthy donors were obtained by counter?flow centrifugal elutriation (CCE) and bronclioalvcolar lavage, respectively. The LAK activity induced by incubation of blood mononuclear cells (MNC) for 4 days with recombinant interleukin 2 (IL?2) was measured by a 4?h 51Cr release assay. The LAK cells were not cytotoxic to freshly isolated monocytes, but were cytotoxic to autologous fresh AM and monocytes that had been incubated for more than 4 days in medium alone. Blood monocytes that had been incubated for 4? days in medium with granulocyte?macrophage colony?stimulating factor (GM?CSF), macrophage colony?stimulating factor (M?CSF) or interleukin 3 (IL?3) were much more susceptible than untreated monocytes to the cytotoxicity of LAK cells. When blood monocytes were separated by CCE into subpopulations of three sizes (small, medium and large), the medium? and large?sized monocytes showed greater responses to GM?CSF in terms of DNA synthesis and colony formation than the small?sized cells. After treatment with GM?CSF for 4 days, these medium and large monocytes were more susceptible than the small monocytes to the cytotoxic action of LAK cells. These results suggest that LAK cells may be important in situ in down?regulating the functions of mature macrophages and blood monocytes that have responded to GM?CSF. Twit Text FOAVip Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte Macrophage Colony stimulating Factor by Human Lymphokine activated Killer Cells ????Jpn J Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=2507489 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2507489 #FOAvip English Wikipedia <ref>{{Cite pmid|2507489}}</ref> Killing of Alveolar Macrophages and of Monocytes that Have Responded to Granulocyte?Macrophage Colony?stimulating Factor by Human Lymphokine?activated Killer Cells #MMPMID2507489 Sone S; Inamura N; Singh SM; Okubo A; Yanagawa H; Nakanishi M; Ogura T Jpn J Cancer Res 1989[Jul]; 80 (7): 662-9 PMID2507489show ga The susceptibilities of human blood monocytes and alveolar macrophages (AM) to cytotoxicity mediated by lymphokine (IL?l)?activated killer (LAK) cells were examined. Monocytes and AM of healthy donors were obtained by counter?flow centrifugal elutriation (CCE) and bronclioalvcolar lavage, respectively. The LAK activity induced by incubation of blood mononuclear cells (MNC) for 4 days with recombinant interleukin 2 (IL?2) was measured by a 4?h 51Cr release assay. The LAK cells were not cytotoxic to freshly isolated monocytes, but were cytotoxic to autologous fresh AM and monocytes that had been incubated for more than 4 days in medium alone. Blood monocytes that had been incubated for 4? days in medium with granulocyte?macrophage colony?stimulating factor (GM?CSF), macrophage colony?stimulating factor (M?CSF) or interleukin 3 (IL?3) were much more susceptible than untreated monocytes to the cytotoxicity of LAK cells. When blood monocytes were separated by CCE into subpopulations of three sizes (small, medium and large), the medium? and large?sized monocytes showed greater responses to GM?CSF in terms of DNA synthesis and colony formation than the small?sized cells. After treatment with GM?CSF for 4 days, these medium and large monocytes were more susceptible than the small monocytes to the cytotoxic action of LAK cells. These results suggest that LAK cells may be important in situ in down?regulating the functions of mature macrophages and blood monocytes that have responded to GM?CSF. äKilling of Alveolar Macrophages and of Monocytes that Have Responded t(epmc).pdf DeepDyve Pubget Overpricing