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1989 ; 80
(1
): 24-30
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Biochemical and immunological characterization of murine leukemia viruses that
are paralysis-inducing in rats
#MMPMID2540131
Shibamura S
; Kai K
; Akatsuka T
; Odaka T
Jpn J Cancer Res
1989[Jan]; 80
(1
): 24-30
PMID2540131
show ga
The molecular size and pI of the viral structural proteins of four PVC viruses
(PVC111, PVC211, PVC321 and PVC441) were compared by single or two-dimensional
polyacrylamide gel electrophoresis. PVC111 had slightly larger p15E and gPr85
molecules (about 0.5 kilodalton) than did the other PVC viruses. On the other
hand, the virion structural proteins p30, p15, p12E and p12 from all the viruses
had the same molecular sizes and pIs. The gp70s and p10s from all the viruses
showed the same molecular sizes. A monoclonal antibody to gp70 of PVC321 virus
recognized the gp70s of all PVC viruses, but not the gp70s of four clones of the
wild mouse ecotropic viruses, Friend murine leukemia viruses (F-MuLV), AKR
ecotropic MuLV, dual-tropic F-MuLV or NZB endogenous xenotropic MuLV, revealing
that these four PVC viruses are homologous with each other, but distinct from the
known mouse retroviruses.