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1988 ; 79
(8
): 952-6
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Detection of gangliosides as N-glycolylneuraminic acid-specific tumor-associated
Hanganutziu-Deicher antigen in human retinoblastoma cells
#MMPMID2460424
Higashi H
; Sasabe T
; Fukui Y
; Maru M
; Kato S
Jpn J Cancer Res
1988[Aug]; 79
(8
): 952-6
PMID2460424
show ga
Gangliosides were shown to bear the tumor-associated N-glycolylneuraminic acid
(NeuGc)-specific Hanganutziu-Deicher (HD) antigen expressed in human
retinoblastoma cells. HD antigenic gangliosides were detected by thin-layer
chromatography/enzyme-immunostaining using affinity-purified chicken antibody
against GM3 containing NeuGc and horseradish peroxidase-conjugated anti-chicken
IgG. One to four species of the antigenic gangliosides were detected from all of
4 cell lines, Y79, WERI-Rb1, TOTL1, and YK, as well as freshly cultured
retinoblastoma cells and isolated tumor tissue. All cases contained GM3(NeuGc) as
an HD antigen. No HD antigenic ganglioside was detected in normal retinal tissues
by the same procedure.