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2018 ; 9
(10
): 2791-2796
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Mass and charge distributions of amyloid fibers involved in neurodegenerative
diseases: mapping heterogeneity and polymorphism
#MMPMID29732065
Pansieri J
; Halim MA
; Vendrely C
; Dumoulin M
; Legrand F
; Sallanon MM
; Chierici S
; Denti S
; Dagany X
; Dugourd P
; Marquette C
; Antoine R
; Forge V
Chem Sci
2018[Mar]; 9
(10
): 2791-2796
PMID29732065
show ga
Heterogeneity and polymorphism are generic features of amyloid fibers with some
important effects on the related disease development. We report here the
characterization, by charge detection mass spectrometry, of amyloid fibers made
of three polypeptides involved in neurodegenerative diseases: A?(1-42) peptide,
tau and ?-synuclein. Beside the mass of individual fibers, this technique enables
to characterize the heterogeneity and the polymorphism of the population. In the
case of A?(1-42) peptide and tau protein, several coexisting species could be
distinguished and characterized. In the case of ?-synuclein, we show how the
polymorphism affects the mass and charge distributions.