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10.1038/s41598-018-24033-7

http://scihub22266oqcxt.onion/10.1038/s41598-018-24033-7
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C5913229!5913229!29686251
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suck abstract from ncbi


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pmid29686251      Sci+Rep 2018 ; 8 (ä): ä
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  • Example-Based Super-Resolution Fluorescence Microscopy #MMPMID29686251
  • Jia S; Han B; Kutz JN
  • Sci Rep 2018[]; 8 (ä): ä PMID29686251show ga
  • Capturing biological dynamics with high spatiotemporal resolution demands the advancement in imaging technologies. Super-resolution fluorescence microscopy offers spatial resolution surpassing the diffraction limit to resolve near-molecular-level details. While various strategies have been reported to improve the temporal resolution of super-resolution imaging, all super-resolution techniques are still fundamentally limited by the trade-off associated with the longer image acquisition time that is needed to achieve higher spatial information. Here, we demonstrated an example-based, computational method that aims to obtain super-resolution images using conventional imaging without increasing the imaging time. With a low-resolution image input, the method provides an estimate of its super-resolution image based on an example database that contains super- and low-resolution image pairs of biological structures of interest. The computational imaging of cellular microtubules agrees approximately with the experimental super-resolution STORM results. This new approach may offer potential improvements in temporal resolution for experimental super-resolution fluorescence microscopy and provide a new path for large-data aided biomedical imaging.
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