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10.1016/j.kint.2017.12.003 http://scihub22266oqcxt.onion/10.1016/j.kint.2017.12.003 C5912930!5912930 !29433915     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29433915 &cmd=llinks): Failed to open stream: HTTP request failed! 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A novel assay provides sensitive measurement of physiologically relevant changes in albumin permeability in isolated human and rodent glomeruli |pdf=|usr=}}{{29433915 }} gab.com Text A novel assay provides sensitive measurement of physiologically relevant changes in albumin permeability in isolated human and rodent glomeruli JO: Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=29433915 #FOAvip Increased urinary albumin excretion is a key feature of glomerular disease but has limitations as a measure of glomerular permeability. Here we describe a novel assay to measure the apparent albumin permeability of single capillaries in glomeruli, isolated from perfused kidneys cleared of red blood cells. The rate of decline of the albumin concentration within the capillary lumen was quantified using confocal microscopy and used to calculate apparent permeability. The assay was extensively validated and provided robust, reproducible estimates of glomerular albumin permeability. These values were comparable with previous in vivo data, showing this assay could be applied to human as well as rodent glomeruli. To confirm this, we showed that targeted endothelial glycocalyx disruption resulted in increased glomerular albumin permeability in mice. Furthermore, incubation with plasma from patients with post-transplant recurrence of nephrotic syndrome increased albumin permeability in rat glomeruli compared to remission plasma. Finally, in glomeruli isolated from rats with early diabetes there was a significant increase in albumin permeability and loss of endothelial glycocalyx, both of which were ameliorated by angiopoietin-1. Thus, a glomerular permeability assay, producing physiologically relevant values with sufficient sensitivity to measure changes in glomerular permeability and independent of tubular function, was developed and validated. This assay significantly advances the ability to study biology and disease in rodent and human glomeruli. Twit Text FOAVip A novel assay provides sensitive measurement of physiologically relevant changes in albumin permeability in isolated human and rodent glomeruli ????Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=29433915 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29433915 #FOAvip English Wikipedia <ref>{{Cite pmid|29433915 }}</ref> A novel assay provides sensitive measurement of physiologically relevant changes in albumin permeability in isolated human and rodent glomeruli #MMPMID29433915 Desideri S ; Onions KL ; Qiu Y ; Ramnath RD ; Butler MJ ; Neal CR ; King MLR ; Salmon AE ; Saleem MA ; Welsh GI ; Michel CC ; Satchell SC ; Salmon AHJ ; Foster RR Kidney Int 2018[May]; 93 (5 ): 1086-1097 PMID29433915 show ga Increased urinary albumin excretion is a key feature of glomerular disease but has limitations as a measure of glomerular permeability. Here we describe a novel assay to measure the apparent albumin permeability of single capillaries in glomeruli, isolated from perfused kidneys cleared of red blood cells. The rate of decline of the albumin concentration within the capillary lumen was quantified using confocal microscopy and used to calculate apparent permeability. The assay was extensively validated and provided robust, reproducible estimates of glomerular albumin permeability. These values were comparable with previous in vivo data, showing this assay could be applied to human as well as rodent glomeruli. To confirm this, we showed that targeted endothelial glycocalyx disruption resulted in increased glomerular albumin permeability in mice. Furthermore, incubation with plasma from patients with post-transplant recurrence of nephrotic syndrome increased albumin permeability in rat glomeruli compared to remission plasma. Finally, in glomeruli isolated from rats with early diabetes there was a significant increase in albumin permeability and loss of endothelial glycocalyx, both of which were ameliorated by angiopoietin-1. Thus, a glomerular permeability assay, producing physiologically relevant values with sufficient sensitivity to measure changes in glomerular permeability and independent of tubular function, was developed and validated. This assay significantly advances the ability to study biology and disease in rodent and human glomeruli. |*Capillary Permeability/drug effects [MESH] |Albuminuria/metabolism/physiopathology [MESH] |Angiopoietin-1/pharmacology [MESH] |Animals [MESH] |Biological Assay/*methods [MESH] |Capillaries/drug effects/*metabolism/physiopathology [MESH] |Diabetes Mellitus, Experimental/metabolism/physiopathology [MESH] |Diabetes Mellitus, Type 1/metabolism/physiopathology [MESH] |Female [MESH] |Glycocalyx/metabolism [MESH] |Humans [MESH] |In Vitro Techniques [MESH] |Kidney Glomerulus/*blood supply [MESH] |Kinetics [MESH] |Male [MESH] |Mice, Inbred C57BL [MESH] |Microscopy, Confocal [MESH] |Nephrotic Syndrome/blood/physiopathology [MESH] |Rats, Sprague-Dawley [MESH] |Reproducibility of Results [MESH]A novel assay provides sensitive measurement of physiologically releva(epmc).pdf DeepDyve Pubget Overpricing