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10.7554/eLife.33572 http://scihub22266oqcxt.onion/10.7554/eLife.33572 C5910022!5910022 !29596046     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29596046 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Elife 2018 ; 7 (ä): ä Nephropedia Template TP {{tp|p=29596046 |t=2018. Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme |pdf=|usr=}}{{29596046 }} gab.com Text Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=29596046 #FOAvip Insulin degrading enzyme (IDE) plays key roles in degrading peptides vital in type two diabetes, Alzheimer's, inflammation, and other human diseases. However, the process through which IDE recognizes peptides that tend to form amyloid fibrils remained unsolved. We used cryoEM to understand both the apo- and insulin-bound dimeric IDE states, revealing that IDE displays a large opening between the homologous ~55 kDa N- and C-terminal halves to allow selective substrate capture based on size and charge complementarity. We also used cryoEM, X-ray crystallography, SAXS, and HDX-MS to elucidate the molecular basis of how amyloidogenic peptides stabilize the disordered IDE catalytic cleft, thereby inducing selective degradation by substrate-assisted catalysis. Furthermore, our insulin-bound IDE structures explain how IDE processively degrades insulin by stochastically cutting either chain without breaking disulfide bonds. Together, our studies provide a mechanism for how IDE selectively degrades amyloidogenic peptides and offers structural insights for developing IDE-based therapies. Twit Text FOAVip Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=29596046 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29596046 #FOAvip English Wikipedia <ref>{{Cite pmid|29596046 }}</ref> Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme #MMPMID29596046 Zhang Z ; Liang WG ; Bailey LJ ; Tan YZ ; Wei H ; Wang A ; Farcasanu M ; Woods VA ; McCord LA ; Lee D ; Shang W ; Deprez-Poulain R ; Deprez B ; Liu DR ; Koide A ; Koide S ; Kossiakoff AA ; Li S ; Carragher B ; Potter CS ; Tang WJ Elife 2018[Mar]; 7 (ä): ä PMID29596046 show ga Insulin degrading enzyme (IDE) plays key roles in degrading peptides vital in type two diabetes, Alzheimer's, inflammation, and other human diseases. However, the process through which IDE recognizes peptides that tend to form amyloid fibrils remained unsolved. We used cryoEM to understand both the apo- and insulin-bound dimeric IDE states, revealing that IDE displays a large opening between the homologous ~55 kDa N- and C-terminal halves to allow selective substrate capture based on size and charge complementarity. We also used cryoEM, X-ray crystallography, SAXS, and HDX-MS to elucidate the molecular basis of how amyloidogenic peptides stabilize the disordered IDE catalytic cleft, thereby inducing selective degradation by substrate-assisted catalysis. Furthermore, our insulin-bound IDE structures explain how IDE processively degrades insulin by stochastically cutting either chain without breaking disulfide bonds. Together, our studies provide a mechanism for how IDE selectively degrades amyloidogenic peptides and offers structural insights for developing IDE-based therapies. |Cryoelectron Microscopy [MESH] |Crystallography, X-Ray [MESH] |Humans [MESH] |Insulin/*chemistry/*metabolism [MESH] |Insulysin/*chemistry/*metabolism [MESH] |Mass Spectrometry [MESH] |Protein Binding [MESH] |Protein Conformation [MESH] |Protein Multimerization [MESH] |Proteolysis [MESH]Ensemble cryoEM elucidates the mechanism of insulin capture and degrad(epmc).pdf DeepDyve Pubget Overpricing