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10.1091/mbc.E17-05-0288

http://scihub22266oqcxt.onion/10.1091/mbc.E17-05-0288
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C5909934!5909934!29167381
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suck abstract from ncbi


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pmid29167381      Mol+Biol+Cell 2018 ; 29 (2): 75-83
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  • CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells #MMPMID29167381
  • Reber S; Mechtersheimer J; Nasif S; Benitez JA; Colombo M; Domanski M; Jutzi D; Hedlund E; Ruepp MD
  • Mol Biol Cell 2018[Jan]; 29 (2): 75-83 PMID29167381show ga
  • Conventional nonhomologous end joining?based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents the expression of the open reading frame, resulting in clean gene knockouts.
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