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10.1002/advs.201700731

http://scihub22266oqcxt.onion/10.1002/advs.201700731
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C5908352!5908352!29721420
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suck abstract from ncbi


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pmid29721420      Adv+Sci+(Weinh) 2018 ; 5 (4): ä
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  • A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells #MMPMID29721420
  • Cools J; Jin Q; Yoon E; Alba Burbano D; Luo Z; Cuypers D; Callewaert G; Braeken D; Gracias DH
  • Adv Sci (Weinh) 2018[Apr]; 5 (4): ä PMID29721420show ga
  • Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips for recording from small populations or even single cells, they rely primarily on the interface between the cells and 2D flat electrodes. Here, an approach that utilizes residual stress?based self?folding to create individually addressable multielectrode interfaces that wrap around the cell in 3D and function as an electrical shell?like recording device is described. These devices are optically transparent, allowing for simultaneous fluorescence imaging. Cell viability is maintained during and after electrode wrapping around the cel and chemicals can diffuse into and out of the self?folding devices. It is further shown that 3D spatiotemporal recordings are possible and that the action potentials recorded from cultured neonatal rat ventricular cardiomyocytes display significantly higher signal?to?noise ratios in comparison with signals recorded with planar extracellular electrodes. It is anticipated that this device can provide the foundation for the development of new?generation MEAs where dynamic electrode?cell interfacing and recording substitutes the traditional method using static electrodes.
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