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10.1161/JAHA.117.007572 http://scihub22266oqcxt.onion/10.1161/JAHA.117.007572 C5907547!5907547!29514810     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 320.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 320.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 320.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Heart+Assoc 2018 ; 7 (6): ä Nephropedia Template TP {{tp|p=29514810|t=2018. Phosphatidylinositol 3?Kinase?DNA Methyltransferase 1?miR?1281?Histone Deacetylase 4 Regulatory Axis Mediates Platelet?Derived Growth Factor?Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells |pdf=|usr=}}{{29514810}} gab.com Text Phosphatidylinositol 3 Kinase DNA Methyltransferase 1 miR 1281 Histone Deacetylase 4 Regulatory Axis Mediates Platelet Derived Growth Factor Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells JO: J Am Heart Assoc http://www.kidney.de/pget.php?&tw=1&pmid=29514810 #FOAvip Background: Platelet?derived growth factor BB, a potent mitogen of pulmonary artery smooth muscle cells (PASMCs), has been implicated in pulmonary arterial remodeling, which is a key pathogenic feature of pulmonary arterial hypertension. Previous microRNA profiling in platelet?derived growth factor BB?treated PASMCs found a significantly downregulated microRNA, miR?1281, but it has not been associated with any cellular function, and we investigated the possibility. Methods and Results: Real?time quantitative reverse transcription?polymerase chain reaction assay proved that downregulation of miR?1281 was a conserved phenomenon in human and rat PASMCs. Overexpression and inhibition of miR?1281 in PASMCs promoted and suppressed, respectively, the cell proliferation and migration. Bioinformatic prediction and 3??untranslated region reporter assay identified histone deacetylase 4 to be a direct target of miR?1281. Supporting this, proliferation and migration assay demonstrated the cellular function of histone deacetylase 4 is inversely correlated with that of miR?1281. Mechanistically, it is found that platelet?derived growth factor BB activates the phosphatidylinositol 3?kinase pathway, which then induces the expression of DNA methyltransferase 1, leading to enhanced methylation of a flanking CpG island and repressed miR?1281 expression. Finally, a reduced miR?1281 level was consistently identified in hypoxic PASMCs in vitro, in pulmonary arteries of rats with monocrotaline?induced pulmonary arterial hypertension, and in serum of patients with coronary heart disease?pulmonary arterial hypertension. These data suggest that there may be a diagnostic and therapeutic use for miR?1281. Conclusions: Herein, we report a novel regulatory axis, phosphatidylinositol 3?kinase?DNA methyltransferase 1?miR?1281?histone deacetylase 4, integrating multiple epigenetic regulators that participate in platelet?derived growth factor BB?stimulated PASMC proliferation and migration and pulmonary vascular remodeling. Twit Text FOAVip Phosphatidylinositol 3 Kinase DNA Methyltransferase 1 miR 1281 Histone Deacetylase 4 Regulatory Axis Mediates Platelet Derived Growth Factor Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells ????J Am Heart Assoc http://www.kidney.de/pget.php?&tw=1&pmid=29514810 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29514810 #FOAvip English Wikipedia <ref>{{Cite pmid|29514810}}</ref> Phosphatidylinositol 3?Kinase?DNA Methyltransferase 1?miR?1281?Histone Deacetylase 4 Regulatory Axis Mediates Platelet?Derived Growth Factor?Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells #MMPMID29514810 Li Y; Li L; Qian Z; Lin B; Chen J; Luo Y; Qu J; Raj JU; Gou D J Am Heart Assoc 2018[Mar]; 7 (6): ä PMID29514810show ga Background: Platelet?derived growth factor BB, a potent mitogen of pulmonary artery smooth muscle cells (PASMCs), has been implicated in pulmonary arterial remodeling, which is a key pathogenic feature of pulmonary arterial hypertension. Previous microRNA profiling in platelet?derived growth factor BB?treated PASMCs found a significantly downregulated microRNA, miR?1281, but it has not been associated with any cellular function, and we investigated the possibility. Methods and Results: Real?time quantitative reverse transcription?polymerase chain reaction assay proved that downregulation of miR?1281 was a conserved phenomenon in human and rat PASMCs. Overexpression and inhibition of miR?1281 in PASMCs promoted and suppressed, respectively, the cell proliferation and migration. Bioinformatic prediction and 3??untranslated region reporter assay identified histone deacetylase 4 to be a direct target of miR?1281. Supporting this, proliferation and migration assay demonstrated the cellular function of histone deacetylase 4 is inversely correlated with that of miR?1281. Mechanistically, it is found that platelet?derived growth factor BB activates the phosphatidylinositol 3?kinase pathway, which then induces the expression of DNA methyltransferase 1, leading to enhanced methylation of a flanking CpG island and repressed miR?1281 expression. Finally, a reduced miR?1281 level was consistently identified in hypoxic PASMCs in vitro, in pulmonary arteries of rats with monocrotaline?induced pulmonary arterial hypertension, and in serum of patients with coronary heart disease?pulmonary arterial hypertension. These data suggest that there may be a diagnostic and therapeutic use for miR?1281. Conclusions: Herein, we report a novel regulatory axis, phosphatidylinositol 3?kinase?DNA methyltransferase 1?miR?1281?histone deacetylase 4, integrating multiple epigenetic regulators that participate in platelet?derived growth factor BB?stimulated PASMC proliferation and migration and pulmonary vascular remodeling. äPhosphatidylinositol 3?Kinase?DNA Methyltransferase 1?miR?1281?Histone(epmc).pdf DeepDyve Pubget Overpricing