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10.1038/nchembio.2559 http://scihub22266oqcxt.onion/10.1038/nchembio.2559 C5902734!5902734!29377001     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2018 ; 14 (3): 311-6 Nephropedia Template TP {{tp|p=29377001|t=2018. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity |pdf=|usr=}}{{29377001}} gab.com Text Partial DNA-guided Cas9 enables genome editing with reduced off-target activity JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=29377001 #FOAvip CRISPR?Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both crRNA and sgRNA, as well as with multiple guide sequences. Partial DNA replacement also works for crRNA of Cpf1, another CRISPR system. We find that partial DNA replacement in the guide sequence significantly reduces off-target genome editing through focused analysis of off-target cleavage, measurement of mismatch tolerance and genome-wide profiling of off-target sites. Using the structure of the Cas9?sgRNA complex as a guide, the majority of the 3? end of crRNA can be replaced with DNA nucleotide, and the 5 - and 3?-DNA-replaced crRNA enables efficient genome editing. Cas9 guided by a DNA?RNA chimera may provide a generalized strategy to reduce both the cost and the off-target genome editing in human cells. Twit Text FOAVip Partial DNA-guided Cas9 enables genome editing with reduced off-target activity ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=29377001 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29377001 #FOAvip English Wikipedia <ref>{{Cite pmid|29377001}}</ref> Partial DNA-guided Cas9 enables genome editing with reduced off-target activity #MMPMID29377001 Yin H; Song CQ; Suresh S; Kwan SY; Wu Q; Walsh S; Ding J; Bogorad RL; Zhu LJ; Wolfe SA; Koteliansky V; Xue W; Langer R; Anderson DG Nat Chem Biol 2018[Mar]; 14 (3): 311-6 PMID29377001show ga CRISPR?Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both crRNA and sgRNA, as well as with multiple guide sequences. Partial DNA replacement also works for crRNA of Cpf1, another CRISPR system. We find that partial DNA replacement in the guide sequence significantly reduces off-target genome editing through focused analysis of off-target cleavage, measurement of mismatch tolerance and genome-wide profiling of off-target sites. Using the structure of the Cas9?sgRNA complex as a guide, the majority of the 3? end of crRNA can be replaced with DNA nucleotide, and the 5 - and 3?-DNA-replaced crRNA enables efficient genome editing. Cas9 guided by a DNA?RNA chimera may provide a generalized strategy to reduce both the cost and the off-target genome editing in human cells. äPartial DNA-guided Cas9 enables genome editing with reduced off-target(epmc).pdf DeepDyve Pubget Overpricing