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10.1007/978-1-4939-2569-8_22 http://scihub22266oqcxt.onion/10.1007/978-1-4939-2569-8_22 C5901726!5901726!25800849     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2015 ; 1298 (ä): 259-70 Nephropedia Template TP {{tp|p=25800849|t=2015. Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay |pdf=|usr=}}{{25800849}} gab.com Text Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25800849 #FOAvip Rab5 targets to early endosomes and is a master regulator of early endosome fusion and endocytosis in all eukaryotic cells. Like other GTPases, Rab5 functions as a molecular switch by alternating between GTP-bound and GDP-bound forms, with the former being biologically active via interactions with multiple effector proteins. Thus the Rab5-GTP level in the cell reflects Rab5 activity in promoting endosome fusion and endocytosis and is indicative of cellular endocytic activity. In this chapter, we describe a Rab5 activity assay by using GST fusion proteins with the Rab5 effectors such as Rabaptin-5, Rabenosyn-5, and EEA1 that specifically bind to GTP-bound Rab5. We compare the efficiencies of the three GST fusion proteins in the pull-down of mammalian and fungal Rab5 proteins. Twit Text FOAVip Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25800849 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25800849 #FOAvip English Wikipedia <ref>{{Cite pmid|25800849}}</ref> Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay #MMPMID25800849 Qi Y; Liang Z; Wang Z; Lu G; Li G Methods Mol Biol 2015[]; 1298 (ä): 259-70 PMID25800849show ga Rab5 targets to early endosomes and is a master regulator of early endosome fusion and endocytosis in all eukaryotic cells. Like other GTPases, Rab5 functions as a molecular switch by alternating between GTP-bound and GDP-bound forms, with the former being biologically active via interactions with multiple effector proteins. Thus the Rab5-GTP level in the cell reflects Rab5 activity in promoting endosome fusion and endocytosis and is indicative of cellular endocytic activity. In this chapter, we describe a Rab5 activity assay by using GST fusion proteins with the Rab5 effectors such as Rabaptin-5, Rabenosyn-5, and EEA1 that specifically bind to GTP-bound Rab5. We compare the efficiencies of the three GST fusion proteins in the pull-down of mammalian and fungal Rab5 proteins. äDetermination of Rab5 Activity in the Cell by Effector Pull-Down Assay(epmc).pdf DeepDyve Pubget Overpricing