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10.1038/s41467-018-03731-w http://scihub22266oqcxt.onion/10.1038/s41467-018-03731-w C5899147!5899147!29654229     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2018 ; 9 (ä): ä Nephropedia Template TP {{tp|p=29654229|t=2018. Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling |pdf=|usr=}}{{29654229}} gab.com Text Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29654229 #FOAvip To accurately recapitulate the heterogeneity of human diseases, animal models require to recreate multiple complex genetic alterations. Here, we combine the RCAS-TVA system with the CRISPR-Cas9 genome editing tools for precise modeling of human tumors. We show that somatic deletion in neural stem cells of a variety of known tumor suppressor genes (Trp53, Cdkn2a, and Pten) leads to high-grade glioma formation. Moreover, by simultaneous delivery of pairs of guide RNAs we generate different gene fusions with oncogenic potential, either by chromosomal deletion (Bcan-Ntrk1) or by chromosomal translocation (Myb-Qk). Lastly, using homology-directed-repair, we also produce tumors carrying the homologous mutation to human BRAF V600E, frequently identified in a variety of tumors, including different types of gliomas. In summary, we have developed an extremely versatile mouse model for in vivo somatic genome editing, that will elicit the generation of more accurate cancer models particularly appropriate for pre-clinical testing. Twit Text FOAVip Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29654229 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29654229 #FOAvip English Wikipedia <ref>{{Cite pmid|29654229}}</ref> Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling #MMPMID29654229 Oldrini B; Curiel-García Á; Marques C; Matia V; Uluçkan Ö; Graña-Castro O; Torres-Ruiz R; Rodriguez-Perales S; Huse JT; Squatrito M Nat Commun 2018[]; 9 (ä): ä PMID29654229show ga To accurately recapitulate the heterogeneity of human diseases, animal models require to recreate multiple complex genetic alterations. Here, we combine the RCAS-TVA system with the CRISPR-Cas9 genome editing tools for precise modeling of human tumors. We show that somatic deletion in neural stem cells of a variety of known tumor suppressor genes (Trp53, Cdkn2a, and Pten) leads to high-grade glioma formation. Moreover, by simultaneous delivery of pairs of guide RNAs we generate different gene fusions with oncogenic potential, either by chromosomal deletion (Bcan-Ntrk1) or by chromosomal translocation (Myb-Qk). Lastly, using homology-directed-repair, we also produce tumors carrying the homologous mutation to human BRAF V600E, frequently identified in a variety of tumors, including different types of gliomas. In summary, we have developed an extremely versatile mouse model for in vivo somatic genome editing, that will elicit the generation of more accurate cancer models particularly appropriate for pre-clinical testing. äSomatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precis(epmc).pdf DeepDyve Pubget Overpricing