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10.1038/nprot.2017.088 http://scihub22266oqcxt.onion/10.1038/nprot.2017.088 C5898606!5898606!28933777     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2017 ; 12 (10): 2169-88 Nephropedia Template TP {{tp|p=28933777|t=2017. Generation and use of a humanized bone marrow ossicle niche for hematopoietic xenotransplantation into mice |pdf=|usr=}}{{28933777}} gab.com Text Generation and use of a humanized bone marrow ossicle niche for hematopoietic xenotransplantation into mice JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28933777 #FOAvip Xenotransplantation is frequently used to study normal and malignant hematopoiesis of human cells. However, conventional xenotransplantation mouse models lack essential human-specific bone marrow (BM) microenvironment-derived survival, proliferation, and self-renewal signals for engraftment of normal and malignant blood cells. As a consequence, many human leukemias and other hematologic disorders do not robustly engraft in these conventional models. Here, we describe a complete workflow for the generation of humanized ossicles with an accessible BM microenvironment that faithfully recapitulates normal BM niche morphology and function. The ossicles therefore allow for accelerated and superior engraftment of primary patient-derived acute myeloid leukemia (AML) and other hematologic malignancies such as myelofibrosis (MF) in mice. The humanized ossicles are formed by in situ differentiation of BM-derived mesenchymal stromal cells (MSCs). Human hematopoietic cells can subsequently be transplanted directly into the ossicle marrow space or via intravenous injection. Using this method, a humanized engraftable BM microenvironment can be formed within 6 ? 10 weeks. Engraftment of human hematopoietic cells can be evaluated by flow cytometry 8 ? 16 weeks after transplantation. This protocol describes a robust and reproducible in vivo methodology to study human normal and malignant hematopoiesis in a more physiologic setting. Twit Text FOAVip Generation and use of a humanized bone marrow ossicle niche for hematopoietic xenotransplantation into mice ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28933777 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28933777 #FOAvip English Wikipedia <ref>{{Cite pmid|28933777}}</ref> Generation and use of a humanized bone marrow ossicle niche for hematopoietic xenotransplantation into mice #MMPMID28933777 Reinisch A; Hernandez DC; Schallmoser K; Majeti R Nat Protoc 2017[Oct]; 12 (10): 2169-88 PMID28933777show ga Xenotransplantation is frequently used to study normal and malignant hematopoiesis of human cells. However, conventional xenotransplantation mouse models lack essential human-specific bone marrow (BM) microenvironment-derived survival, proliferation, and self-renewal signals for engraftment of normal and malignant blood cells. As a consequence, many human leukemias and other hematologic disorders do not robustly engraft in these conventional models. Here, we describe a complete workflow for the generation of humanized ossicles with an accessible BM microenvironment that faithfully recapitulates normal BM niche morphology and function. The ossicles therefore allow for accelerated and superior engraftment of primary patient-derived acute myeloid leukemia (AML) and other hematologic malignancies such as myelofibrosis (MF) in mice. The humanized ossicles are formed by in situ differentiation of BM-derived mesenchymal stromal cells (MSCs). Human hematopoietic cells can subsequently be transplanted directly into the ossicle marrow space or via intravenous injection. Using this method, a humanized engraftable BM microenvironment can be formed within 6 ? 10 weeks. Engraftment of human hematopoietic cells can be evaluated by flow cytometry 8 ? 16 weeks after transplantation. This protocol describes a robust and reproducible in vivo methodology to study human normal and malignant hematopoiesis in a more physiologic setting. äGeneration and use of a humanized bone marrow ossicle niche for hemato(epmc).pdf DeepDyve Pubget Overpricing