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10.1093/jmicro/dfv366 http://scihub22266oqcxt.onion/10.1093/jmicro/dfv366 C5895108!5895108!26611544     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microscopy+(Oxf) 2016 ; 65 (1): 9-22 Nephropedia Template TP {{tp|p=26611544|t=2016. Single-particle cryo-electron microscopy of macromolecular complexes |pdf=|usr=}}{{26611544}} gab.com Text Single-particle cryo-electron microscopy of macromolecular complexes JO: Microscopy (Oxf) http://www.kidney.de/pget.php?&tw=1&pmid=26611544 #FOAvip Recent technological breakthroughs in image acquisition have enabled single-particle cryo-electron microscopy (cryo-EM) to achieve near-atomic resolution structural information for biological complexes. The improvements in image quality coupled with powerful computational methods for sorting distinct particle populations now also allow the determination of compositional and conformational ensembles, thereby providing key insights into macromolecular function. However, the inherent instability and dynamic nature of biological assemblies remain a tremendous challenge that often requires tailored approaches for successful implementation of the methodology. Here, we briefly describe the fundamentals of single-particle cryo-EM with an emphasis on covering the breadth of techniques and approaches, including low- and high-resolution methods, aiming to illustrate specific steps that are crucial for obtaining structural information by this method. Twit Text FOAVip Single-particle cryo-electron microscopy of macromolecular complexes ????Microscopy (Oxf) http://www.kidney.de/pget.php?&tw=1&pmid=26611544 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26611544 #FOAvip English Wikipedia <ref>{{Cite pmid|26611544}}</ref> Single-particle cryo-electron microscopy of macromolecular complexes #MMPMID26611544 Skiniotis G; Southworth DR Microscopy (Oxf) 2016[Feb]; 65 (1): 9-22 PMID26611544show ga Recent technological breakthroughs in image acquisition have enabled single-particle cryo-electron microscopy (cryo-EM) to achieve near-atomic resolution structural information for biological complexes. The improvements in image quality coupled with powerful computational methods for sorting distinct particle populations now also allow the determination of compositional and conformational ensembles, thereby providing key insights into macromolecular function. However, the inherent instability and dynamic nature of biological assemblies remain a tremendous challenge that often requires tailored approaches for successful implementation of the methodology. Here, we briefly describe the fundamentals of single-particle cryo-EM with an emphasis on covering the breadth of techniques and approaches, including low- and high-resolution methods, aiming to illustrate specific steps that are crucial for obtaining structural information by this method. äSingle-particle cryo-electron microscopy of macromolecular complexes (epmc).pdf DeepDyve Pubget Overpricing