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10.3389/fimmu.2018.00504 http://scihub22266oqcxt.onion/10.3389/fimmu.2018.00504 C5890180!5890180 !29662488     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29662488 &cmd=llinks): Failed to open stream: HTTP request failed! 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Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4(+) and CD8(+) T-Cell Responses |pdf=|usr=}}{{29662488 }} gab.com Text Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4(+) and CD8(+) T-Cell Responses JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29662488 #FOAvip PURPOSE: Injection of an antigen into the anterior chamber of the eye induces a peripheral antigen-specific immune modulation mechanism, known as anterior chamber-associated immune deviation (ACAID). Delayed-type hypersensitivity experiments argue that the subretinal space (SR) of the eye displays properties similar to ACAID. However, no investigation was performed regarding the differential impact of a subretinal antigen injection on peripheral CD4(+) versus CD8(+) T cells, on the potential immune deviation regarding Th profiles, and on the antigen-specificity of the inhibition. A better understanding of these mechanisms is crucial to improve safety and immunomonitoring of ongoing therapeutic approaches targeting the SR. The aim of this study is to characterize the proliferative capacities and cytokine patterns of antigen-specific CD4(+) and CD8(+) T cells after a subretinal injection of antigen in mice. METHODS: Ubiquitously Transcribed tetratricopeptide repeat gene Y-linked (UTY) and DEAD Box polypeptide 3 Y-linked (DBY) peptides which respectively include MHCI- and MHCII-restricted T-cell epitopes of the mouse HY male antigen, were injected into the subretinal space of C57BL/6 female mice. 2?weeks later, these mice were immunized subcutaneously with these peptides and compared to control mice. A week later, T-cell immune responses were analyzed by IFN? ELISpot assays and cytokine measurements (IL-2, IL-4, IL-6, IL-10, IL-13, IL-17a, IFN?, TNF?, GM-CSF, and MCP-1) in the spleen and with proliferation assays in draining lymph nodes. RESULTS: Immune cells from mice that received HY peptides in the SR before immunization, compared with those from control immunized mice, secreted significantly smaller quantities of Th1/Tc1, Th2/Tc2, and Th17/Tc17 cytokines, and HY-specific CD4(+) T cells proliferated less in response to HY peptides. CONCLUSION: Taken together, our data clearly demonstrate that the subretinal injection of HY peptides induces a systemic HY-specific inhibition of conventional Th profiles and CD8(+) T cells. We propose to call this phenomenon SRAII, for subretinal-associated immune inhibition. Twit Text FOAVip Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4(+) and CD8(+) T-Cell Responses ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29662488 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29662488 #FOAvip English Wikipedia <ref>{{Cite pmid|29662488 }}</ref> Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4(+) and CD8(+) T-Cell Responses #MMPMID29662488 Vendomèle J ; Dehmani S ; Khebizi Q ; Galy A ; Fisson S Front Immunol 2018[]; 9 (ä): 504 PMID29662488 show ga PURPOSE: Injection of an antigen into the anterior chamber of the eye induces a peripheral antigen-specific immune modulation mechanism, known as anterior chamber-associated immune deviation (ACAID). Delayed-type hypersensitivity experiments argue that the subretinal space (SR) of the eye displays properties similar to ACAID. However, no investigation was performed regarding the differential impact of a subretinal antigen injection on peripheral CD4(+) versus CD8(+) T cells, on the potential immune deviation regarding Th profiles, and on the antigen-specificity of the inhibition. A better understanding of these mechanisms is crucial to improve safety and immunomonitoring of ongoing therapeutic approaches targeting the SR. The aim of this study is to characterize the proliferative capacities and cytokine patterns of antigen-specific CD4(+) and CD8(+) T cells after a subretinal injection of antigen in mice. METHODS: Ubiquitously Transcribed tetratricopeptide repeat gene Y-linked (UTY) and DEAD Box polypeptide 3 Y-linked (DBY) peptides which respectively include MHCI- and MHCII-restricted T-cell epitopes of the mouse HY male antigen, were injected into the subretinal space of C57BL/6 female mice. 2?weeks later, these mice were immunized subcutaneously with these peptides and compared to control mice. A week later, T-cell immune responses were analyzed by IFN? ELISpot assays and cytokine measurements (IL-2, IL-4, IL-6, IL-10, IL-13, IL-17a, IFN?, TNF?, GM-CSF, and MCP-1) in the spleen and with proliferation assays in draining lymph nodes. RESULTS: Immune cells from mice that received HY peptides in the SR before immunization, compared with those from control immunized mice, secreted significantly smaller quantities of Th1/Tc1, Th2/Tc2, and Th17/Tc17 cytokines, and HY-specific CD4(+) T cells proliferated less in response to HY peptides. CONCLUSION: Taken together, our data clearly demonstrate that the subretinal injection of HY peptides induces a systemic HY-specific inhibition of conventional Th profiles and CD8(+) T cells. We propose to call this phenomenon SRAII, for subretinal-associated immune inhibition. |Animals [MESH] |CD4-Positive T-Lymphocytes/*drug effects/immunology [MESH] |CD8-Positive T-Lymphocytes/*drug effects/immunology [MESH] |Cytokines/immunology [MESH] |Female [MESH] |H-Y Antigen/*administration & dosage [MESH] |Injections, Intraocular [MESH] |Mice, Inbred C57BL [MESH] |Peptides/*administration & dosage [MESH]Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific (epmc).pdf DeepDyve Pubget Overpricing