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10.1093/nar/gkx1316 http://scihub22266oqcxt.onion/10.1093/nar/gkx1316 C5888754!5888754 !29325071     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29325071 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acids+Res 2018 ; 46 (6 ): 3187-3197 Nephropedia Template TP {{tp|p=29325071 |t=2018. Viral suppressors of RNAi employ a rapid screening mode to discriminate viral RNA from cellular small RNA |pdf=|usr=}}{{29325071 }} gab.com Text Viral suppressors of RNAi employ a rapid screening mode to discriminate viral RNA from cellular small RNA JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=29325071 #FOAvip RNA interference (RNAi) is an indispensable mechanism for antiviral defense in insects, including mosquitoes that transmit human diseases. To escape this antiviral defense system, viruses encode suppressors of RNAi that prevent elimination of viral RNAs, and thus ensure efficient virus accumulation. Although the first animal Viral Suppressor of RNAi (VSR) was identified more than a decade ago, the molecular basis of RNAi suppression by these viral proteins remains unclear. Here, we developed a single-molecule fluorescence assay to investigate how VSRs inhibit the recognition of viral RNAs by Dcr-2, a key endoribonuclease enzyme in the RNAi pathway. Using VSRs from three insect RNA viruses (Culex Y virus, Drosophila X virus and Drosophila C virus), we reveal bimodal physical interactions between RNA molecules and VSRs. During initial interactions, these VSRs rapidly discriminate short RNA substrates from long dsRNA. VSRs engage nearly irreversible binding with long dsRNAs, thereby shielding it from recognition by Dcr-2. We propose that the length-dependent switch from rapid screening to irreversible binding reflects the main mechanism by which VSRs distinguish viral dsRNA from cellular RNA species such as microRNAs. Twit Text FOAVip Viral suppressors of RNAi employ a rapid screening mode to discriminate viral RNA from cellular small RNA ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=29325071 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29325071 #FOAvip English Wikipedia <ref>{{Cite pmid|29325071 }}</ref> Viral suppressors of RNAi employ a rapid screening mode to discriminate viral RNA from cellular small RNA #MMPMID29325071 Fareh M ; van Lopik J ; Katechis I ; Bronkhorst AW ; Haagsma AC ; van Rij RP ; Joo C Nucleic Acids Res 2018[Apr]; 46 (6 ): 3187-3197 PMID29325071 show ga RNA interference (RNAi) is an indispensable mechanism for antiviral defense in insects, including mosquitoes that transmit human diseases. To escape this antiviral defense system, viruses encode suppressors of RNAi that prevent elimination of viral RNAs, and thus ensure efficient virus accumulation. Although the first animal Viral Suppressor of RNAi (VSR) was identified more than a decade ago, the molecular basis of RNAi suppression by these viral proteins remains unclear. Here, we developed a single-molecule fluorescence assay to investigate how VSRs inhibit the recognition of viral RNAs by Dcr-2, a key endoribonuclease enzyme in the RNAi pathway. Using VSRs from three insect RNA viruses (Culex Y virus, Drosophila X virus and Drosophila C virus), we reveal bimodal physical interactions between RNA molecules and VSRs. During initial interactions, these VSRs rapidly discriminate short RNA substrates from long dsRNA. VSRs engage nearly irreversible binding with long dsRNAs, thereby shielding it from recognition by Dcr-2. We propose that the length-dependent switch from rapid screening to irreversible binding reflects the main mechanism by which VSRs distinguish viral dsRNA from cellular RNA species such as microRNAs. |*RNA Interference [MESH] |Animals [MESH] |Entomobirnavirus/*genetics [MESH] |Humans [MESH] |MicroRNAs/*genetics/metabolism [MESH] |Protein Binding [MESH] |RNA Viruses/*genetics [MESH] |RNA, Double-Stranded/*genetics/metabolism [MESH] |RNA, Small Interfering/genetics/metabolism [MESH] |RNA, Viral/*genetics/metabolism [MESH] |Sf9 Cells [MESH] |Spodoptera [MESH] |Tumor Necrosis Factor Decoy Receptors/genetics/metabolism [MESH]Viral suppressors of RNAi employ a rapid screening mode to discriminat(epmc).pdf DeepDyve Pubget Overpricing