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10.1002/mnfr.201700917 http://scihub22266oqcxt.onion/10.1002/mnfr.201700917 C5887919!5887919!29292863     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Nutr+Food+Res 2018 ; 62 (5): ä Nephropedia Template TP {{tp|p=29292863|t=2018. Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin?Stimulated Glucose Uptake |pdf=|usr=}}{{29292863}} gab.com Text Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin Stimulated Glucose Uptake JO: Mol Nutr Food Res http://www.kidney.de/pget.php?&tw=1&pmid=29292863 #FOAvip Scope: We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results: EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin?stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin?stimulated 2?deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin?mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2?deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion: EVs released by stressed adipocytes impair insulin action in neighboring adipocytes. Twit Text FOAVip Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin Stimulated Glucose Uptake ????Mol Nutr Food Res http://www.kidney.de/pget.php?&tw=1&pmid=29292863 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29292863 #FOAvip English Wikipedia <ref>{{Cite pmid|29292863}}</ref> Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin?Stimulated Glucose Uptake #MMPMID29292863 Mleczko J; Ortega FJ; Falcon?Perez JM; Wabitsch M; Fernandez?Real JM; Mora S Mol Nutr Food Res 2018[Mar]; 62 (5): ä PMID29292863show ga Scope: We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results: EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin?stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin?stimulated 2?deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin?mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2?deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion: EVs released by stressed adipocytes impair insulin action in neighboring adipocytes. äExtracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Redu(epmc).pdf DeepDyve Pubget Overpricing