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10.1007/978-1-61779-328-8_2 http://scihub22266oqcxt.onion/10.1007/978-1-61779-328-8_2 C5886364!5886364!21913091     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2011 ; 793 (ä): 9-21 Nephropedia Template TP {{tp|p=21913091|t=2011. In Vivo and In Vitro Determination of Cell Death Markers in Neurons |pdf=|usr=}}{{21913091}} gab.com Text In Vivo and In Vitro Determination of Cell Death Markers in Neurons JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21913091 #FOAvip Mitochondria are key regulators of cellular death. The mitochondrial membranes contain essential enzyme complexes for maintaining metabolic homeostasis and meeting the energy requirements of the cell (Tait and Green, Nat Rev Mol Cell Biol 11:621?632, 2010 and Galluzzi et al., Apoptosis 12:803?813, 2007). Thus, any perturbation of outer or inner mitochondrial membranes can lead to disruptions in the normal fluxes of key ions and metabolic proteins (i.e., ADP/ATP exchange), leading to eventual cellular death. In addition to maintaining cellular viability, mitochondria play a critical role in the initiation of programmed cell death. As initiators of the cell death process, key mitochondrial proteins [Cytochrome C (Cyt C) one of the most well-studied among them] are released from the intermembrane space during early cell death events eventually leading to caspase activation.Release of Cyt C is a crucial step during cellular death (Tait and Green, Nat Rev Mol Cell Biol 11:621?632, 2010). Therefore, the measurement of Cyt C release can give vital information about cell death signaling. Immunolabeling against Cyt C can give an easy readout of mitochondrial integrity as well, allowing for simultaneous identification of mitochondrial viability (and/or damage) and initiation of intracellular death processes.In this chapter, we use Cyt C as a dual marker of mitochondrial integrity and cell death and review several protocols to measure Cyt C localization into intact mitochondria and its release into the cytosol. The goal is to offer an array of assays that, combined, provide both qualitative and quantitative analysis of the relationship between mitochondrial viability and activation of an intracellular cell death process. Immunofluorescence, Western blot, and ELISA measurements of Cyt C as are discussed in detail. Twit Text FOAVip In Vivo and In Vitro Determination of Cell Death Markers in Neurons ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21913091 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21913091 #FOAvip English Wikipedia <ref>{{Cite pmid|21913091}}</ref> In Vivo and In Vitro Determination of Cell Death Markers in Neurons #MMPMID21913091 Naniche N; Sau D; Pasinelli P Methods Mol Biol 2011[]; 793 (ä): 9-21 PMID21913091show ga Mitochondria are key regulators of cellular death. The mitochondrial membranes contain essential enzyme complexes for maintaining metabolic homeostasis and meeting the energy requirements of the cell (Tait and Green, Nat Rev Mol Cell Biol 11:621?632, 2010 and Galluzzi et al., Apoptosis 12:803?813, 2007). Thus, any perturbation of outer or inner mitochondrial membranes can lead to disruptions in the normal fluxes of key ions and metabolic proteins (i.e., ADP/ATP exchange), leading to eventual cellular death. In addition to maintaining cellular viability, mitochondria play a critical role in the initiation of programmed cell death. As initiators of the cell death process, key mitochondrial proteins [Cytochrome C (Cyt C) one of the most well-studied among them] are released from the intermembrane space during early cell death events eventually leading to caspase activation.Release of Cyt C is a crucial step during cellular death (Tait and Green, Nat Rev Mol Cell Biol 11:621?632, 2010). Therefore, the measurement of Cyt C release can give vital information about cell death signaling. Immunolabeling against Cyt C can give an easy readout of mitochondrial integrity as well, allowing for simultaneous identification of mitochondrial viability (and/or damage) and initiation of intracellular death processes.In this chapter, we use Cyt C as a dual marker of mitochondrial integrity and cell death and review several protocols to measure Cyt C localization into intact mitochondria and its release into the cytosol. The goal is to offer an array of assays that, combined, provide both qualitative and quantitative analysis of the relationship between mitochondrial viability and activation of an intracellular cell death process. Immunofluorescence, Western blot, and ELISA measurements of Cyt C as are discussed in detail. äIn Vivo and In Vitro Determination of Cell Death Markers in Neurons (epmc).pdf DeepDyve Pubget Overpricing