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2018 ; 35
(2
): 79-82
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Utility of Cytospin and Cell block Technology in Evaluation of Body Fluids and
Urine Samples: A Comparative Study
#MMPMID29643653
Qamar I
; Rehman S
; Mehdi G
; Maheshwari V
; Ansari HA
; Chauhan S
J Cytol
2018[Apr]; 35
(2
): 79-82
PMID29643653
show ga
BACKGROUND: Cytologic examination of body fluids commonly involves the use of
direct or sediment smears, cytocentrifuge preparations, membrane filter
preparations, or cell block sections. Cytospin and cell block techniques are
extremely useful in improving cell yield of thin serous effusions and urine
samples, and ensure high diagnostic efficacy. MATERIALS AND METHODS: We studied
cytospin preparations and cell block sections prepared from 180 samples of body
fluids and urine samples to compare the relative efficiency of cell retrieval,
preservation of cell morphology, ease of application of special stains, and
diagnostic efficacy. Samples were collected and processed to prepare cytospin
smears and cell block sections. RESULTS: We observed that overall, cell yield and
preservation of individual cell morphology were better in cytospin preparations
as compared to cell blocks, while preservation of architectural pattern was
better in cell block sections. The number of suspicious cases also decreased on
cell block sections, with increased detection of malignancy. It was difficult to
prepare cell blocks from urine samples due to low cellularity. CONCLUSIONS:
Cytospin technology is a quick, efficient, and cost-effective method of
increasing cell yield in hypocellular samples, with better preservation of cell
morphology. Cell blocks are better prepared from high cellularity fluids;
however, tissue architecture is better studied, with improved rate of diagnosis
and decrease in ambiguous results. Numerous sections can be prepared from a small
amount of material. Special stains and immunochemical stains can be easily
applied to cell blocks. It also provides a source of archival material.
free
free
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