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2018 ; 8
(2
): 170-178
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Preventive effects of the aqueous extract of Cichorium intybus L flower on
ethylene glycol-induced renal calculi in rats
#MMPMID29632848
Emamiyan MZ
; Vaezi G
; Tehranipour M
; Shahrohkabadi K
; Shiravi A
Avicenna J Phytomed
2018[Mar]; 8
(2
): 170-178
PMID29632848
show ga
OBJECTIVE: Urolithiasis remains a global problem. Despite the availability of
numerous methods, no definite therapeutic agent has been yet introduced for the
prevention or treatment of kidney stones. In this study, we evaluated the
possible preventive effects of aqueous extract of Cichorium intybus L. (chicory)
flowers on ethylene glycol-induced renal calculi in rats. MATERIALS AND METHODS:
A total of 24 Wistar rats were randomly divided into four groups and were treated
for 30 days. Group A received drinking tap water, while groups B, C, and D were
administered with 1% ethylene glycol for induction of calcium oxalate stone
formation. Rats in groups C and D received intraperitoneal injections of the
aqueous extract of chicory flowers (50 and 200 mg/kg, respectively) since the
first day of the experiment. The urine volume, urine pH, and urinary levels of
oxalate, citrate, calcium, uric acid, and creatinine as well as serum levels of
calcium, uric acid, and creatinine were measured. After 30 days, the rats'
kidneys were removed and prepared for histological evaluation of calcium oxalate
deposits. One-way analysis of variance (ANOVA), followed by Tukey's test, was
performed, using SPSS version 20. RESULTS: The number of calcium oxalate crystals
was significantly higher in group B (ethylene glycol-only treated animals),
compared to group A (control), group C (50 mg/kg of aqueous extract), and group D
(200 mg/kg of aqueous extract) (p<0.05). On day 30, the urine level of citrate,
oxalate (p>0.05), and creatinine (p<0.05), as well as urine pH (p<0.05) decreased
in groups C and D, compared to group B. Also, urine calcium level, urine uric
acid (p>0.05), and urine volume (p<0.05) were higher in group D, compared to
group B. In addition, the serum level of calcium, creatinine (p<0.05), and uric
acid (p<0.001) decreased in groups C and D. CONCLUSION: The aqueous extract of
chicory flower (50 mg/kg) could reduce the number of calcium oxalate deposits in
the urine and reduce the level of serum parameters.