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http://scihub22266oqcxt.onion/ C5881880!5881880 !29643724     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29643724 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Vis 2018 ; 24 (ä): 274-285 Nephropedia Template TP {{tp|p=29643724 |t=2018. Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice |pdf=|usr=}}{{29643724 }} gab.com Text Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice JO: Mol Vis http://www.kidney.de/pget.php?&tw=1&pmid=29643724 #FOAvip PURPOSE: To investigate the effect and mechanism of proresolving lipid mediator resolvin D1 (RvD1) on the corneal epithelium and the restoration of mechanical sensation in diabetic mice. METHODS: Type 1 diabetes was induced in mice with intraperitoneal streptozocin injections. The healthy and diabetic mice underwent removal of the central corneal epithelium, and then 100 ng/ml RvD1 or its formyl peptide receptor 2 (FPR2) antagonist WRW4 was used to treat the diabetic mice. Regeneration of the corneal epithelium and nerves was observed with sodium fluorescein staining and whole-mount anti-?3-tubulin fluorescence staining. The inflammatory response level was measured with hematoxylin and eosin staining (inflammatory cell infiltration), enzyme-linked immunosorbent assay (tumor necrosis factor alpha and interleukin-1 beta content), myeloperoxidase activity, and fluorescence staining (macrophage content). The reactive oxygen species (ROS) and glutathione (GSH) levels were examined with incubation with fluorescent probes, and oxidative stress-related protein expression levels were evaluated with fluorescence staining and western blotting. RESULTS: Topical application of RvD1 promoted regeneration of the corneal epithelium in diabetic mice, accompanied by the reactivation of signaling and inflammation resolution related to regeneration of the epithelium. Furthermore, RvD1 directly attenuated the accumulation of ROS and nicotinamide adenine dinucleotide phosphate oxidase 2/4 expression, while RvD1 enhanced GSH synthesis and reactivated the Nrf2-ARE signaling pathway that was impaired in the corneal epithelium in the diabetic mice. More interestingly, topical application of RvD1 promoted regeneration of corneal nerves and completely restored impaired mechanical sensitivity of the cornea in diabetic mice. In addition, the promotion of corneal epithelial wound healing by RvD1 in diabetic mice was abolished by its FPR2 antagonist WRW4. CONCLUSIONS: Topical application of RvD1 promotes corneal epithelial wound healing and the restoration of mechanical sensation in diabetic mice, which may be related to the lipid mediator's regulation of inflammation resolution, the reactivation of regenerative signaling in the epithelium, and the attenuation of oxidative stress. Twit Text FOAVip Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice ????Mol Vis http://www.kidney.de/pget.php?&tw=1&pmid=29643724 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29643724 #FOAvip English Wikipedia <ref>{{Cite pmid|29643724 }}</ref> Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice #MMPMID29643724 Zhang Z ; Hu X ; Qi X ; Di G ; Zhang Y ; Wang Q ; Zhou Q Mol Vis 2018[]; 24 (ä): 274-285 PMID29643724 show ga PURPOSE: To investigate the effect and mechanism of proresolving lipid mediator resolvin D1 (RvD1) on the corneal epithelium and the restoration of mechanical sensation in diabetic mice. METHODS: Type 1 diabetes was induced in mice with intraperitoneal streptozocin injections. The healthy and diabetic mice underwent removal of the central corneal epithelium, and then 100 ng/ml RvD1 or its formyl peptide receptor 2 (FPR2) antagonist WRW4 was used to treat the diabetic mice. Regeneration of the corneal epithelium and nerves was observed with sodium fluorescein staining and whole-mount anti-?3-tubulin fluorescence staining. The inflammatory response level was measured with hematoxylin and eosin staining (inflammatory cell infiltration), enzyme-linked immunosorbent assay (tumor necrosis factor alpha and interleukin-1 beta content), myeloperoxidase activity, and fluorescence staining (macrophage content). The reactive oxygen species (ROS) and glutathione (GSH) levels were examined with incubation with fluorescent probes, and oxidative stress-related protein expression levels were evaluated with fluorescence staining and western blotting. RESULTS: Topical application of RvD1 promoted regeneration of the corneal epithelium in diabetic mice, accompanied by the reactivation of signaling and inflammation resolution related to regeneration of the epithelium. Furthermore, RvD1 directly attenuated the accumulation of ROS and nicotinamide adenine dinucleotide phosphate oxidase 2/4 expression, while RvD1 enhanced GSH synthesis and reactivated the Nrf2-ARE signaling pathway that was impaired in the corneal epithelium in the diabetic mice. More interestingly, topical application of RvD1 promoted regeneration of corneal nerves and completely restored impaired mechanical sensitivity of the cornea in diabetic mice. In addition, the promotion of corneal epithelial wound healing by RvD1 in diabetic mice was abolished by its FPR2 antagonist WRW4. CONCLUSIONS: Topical application of RvD1 promotes corneal epithelial wound healing and the restoration of mechanical sensation in diabetic mice, which may be related to the lipid mediator's regulation of inflammation resolution, the reactivation of regenerative signaling in the epithelium, and the attenuation of oxidative stress. |Administration, Topical [MESH] |Animals [MESH] |Carboxylic Ester Hydrolases/genetics/metabolism [MESH] |Corneal Injuries/complications/*drug therapy/genetics/pathology [MESH] |Diabetes Mellitus, Experimental/complications/*drug therapy/genetics/pathology [MESH] |Docosahexaenoic Acids/antagonists & inhibitors/*pharmacology [MESH] |Epithelium, Corneal/drug effects/metabolism/pathology [MESH] |Gene Expression Regulation [MESH] |Glutathione/agonists/metabolism [MESH] |Interleukin-1beta/genetics/metabolism [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |NADH, NADPH Oxidoreductases/genetics/metabolism [MESH] |NF-E2-Related Factor 2/genetics/metabolism [MESH] |Oligopeptides/pharmacology [MESH] |Optic Nerve/drug effects/metabolism [MESH] |Peroxidase/genetics/metabolism [MESH] |Reactive Oxygen Species/antagonists & inhibitors/metabolism [MESH] |Receptors, Formyl Peptide/antagonists & inhibitors/*genetics/metabolism [MESH] |Regeneration/*drug effects [MESH] |Streptozocin [MESH] |Touch Perception/*drug effects [MESH] |Tumor Necrosis Factor-alpha/genetics/metabolism [MESH]Resolvin D1 promotes corneal epithelial wound healing and restoration (epmc).pdf DeepDyve Pubget Overpricing