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A study on volatile organic compounds emitted by in-vitro lung cancer cultured
cells using gas sensor array and SPME-GCMS
#MMPMID29609557
Thriumani R
; Zakaria A
; Hashim YZH
; Jeffree AI
; Helmy KM
; Kamarudin LM
; Omar MI
; Shakaff AYM
; Adom AH
; Persaud KC
BMC Cancer
2018[Apr]; 18
(1
): 362
PMID29609557
show ga
BACKGROUND: Volatile organic compounds (VOCs) emitted from exhaled breath from
human bodies have been proven to be a useful source of information for early lung
cancer diagnosis. To date, there are still arguable information on the production
and origin of significant VOCs of cancer cells. Thus, this study aims to conduct
in-vitro experiments involving related cell lines to verify the capability of
VOCs in providing information of the cells. METHOD: The performances of e-nose
technology with different statistical methods to determine the best classifier
were conducted and discussed. The gas sensor study has been complemented using
solid phase micro-extraction-gas chromatography mass spectrometry. For this
purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast
cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth
medium. RESULTS: This study successfully provided a list of possible volatile
organic compounds that can be specific biomarkers for lung cancer, even at the
24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus
All-Support Vector Machine classifier, is able to produce high performance in
distinguishing lung cancer from breast cancer cells and normal lung cells.
CONCLUSION: The findings in this work conclude that the specific VOC released
from the cancer cells can act as the odour signature and potentially to be used
as non-invasive screening of lung cancer using gas array sensor devices.
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