Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29402349
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29402349
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Am+Assoc+Lab+Anim+Sci
2018 ; 57
(1
): 30-34
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Evaluation of a Commercially Available Euthanasia Solution as a Voluntarily
Ingested Euthanasia Agent in Laboratory Mice
#MMPMID29402349
Dudley ES
; Boivin GP
J Am Assoc Lab Anim Sci
2018[Jan]; 57
(1
): 30-34
PMID29402349
show ga
All currently accepted methods of euthanasia for laboratory mice involve some
degree of stress, fear, anxiety, or pain. We evaluated the voluntary oral
administration of a euthanasia drug in 99 male and 81 female mice of various
strains. We first explored the palatability of sugar-cookie dough with various
flavorings added. We placed the cookie dough in the cage with an adult mouse and
recorded the amount ingested after 1 h. Mice readily ingested all flavors of
sugar-cookie dough. We then added a euthanasia solution containing pentobarbital
and phenytoin to all flavors of cookie dough and placed a small bolus in the cage
of each mouse or mouse pair. We observed the mice for 1 h for clinical signs of
pentobarbital intoxication and then weighed uneaten dough to determine the dose
of pentobarbital ingested. Palatability declined sharply when euthanasia solution
was present. Mice ingested higher doses of pentobarbital in cookie dough during
the dark phase and after fasting. Ingestion caused ataxia in some mice but was
not sufficient to cause loss of righting reflex, unconsciousness, or death in any
mouse. We successfully identified sugar cookie dough as a drug vehicle that was
readily and rapidly eaten by mice without the need for previous exposure.
Additional research is needed to identify euthanasia compounds for mice that do
not affect the palatability of cookie dough.