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Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Med+Sci+Monit 2018 ; 24 (ä): 1633-41 Nephropedia Template TP
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Specific Inhibitor of Smad3 (SIS3) Attenuates Fibrosis, Apoptosis, and Inflammation in Unilateral Ureteral Obstruction Kidneys by Inhibition of Transforming Growth Factor ? (TGF-?)/Smad3 Signaling #MMPMID29555895
Ji X; Wang H; Wu Z; Zhong X; Zhu M; Zhang Y; Tan R; Liu Y; Li J; Wang L
Med Sci Monit 2018[]; 24 (ä): 1633-41 PMID29555895show ga
Background: Fibrosis is the common pathological feature in most kinds of chronic kidney disease (CKD). TGF-?/Smads signaling is the master pathway regulating kidney fibrosis pathogenesis, in which Smad3 acts as the integrator of various pro-fibrosis signals. In this study, we analyzed the role of SIS3, a specific inhibitor of Smad3, in mouse unilateral ureteral obstruction (UUO) kidneys. Material/Methods: UUO mice were intraperitoneally injected with 0.2 mg/kg/day or 2 mg/kg/day of SIS3 or control saline for 7 days, followed by analysis of structure injury, fibrosis status, inflammation, apoptosis, and TGF-?/Smads signaling activity. Results: Our results indicated that SIS3 treatment dosage-dependently relieved the gross structure injury and tubular necrosis in UUO kidneys. Masson staining, immunohistochemistry, and real-time PCR showed significantly decreased extracellular matrix deposition, fibronectin staining intensity, and RNA levels of collagen I and collagen III in SIS3-treated UUO kidneys. SIS3 treatment also suppressed the activation of myofibroblasts, as evidenced by decreased expression levels of ?-SMA and vimentin in UUO kidneys. The TGF-?/Smads signaling activity analysis showed that SIS3 inhibited the phosphorylation of Smad3 but not Smad2 and decreased the protein level of TGF-?1, suggesting specific inhibition of the TGF-?/Smad3 pathway in UUO kidneys. Furthermore, SIS3 treatment also ameliorated the increased pro-inflammatory TNF-? and COX2 in UUO kidneys and circulating IL-1? in UUO mice, and inhibited caspase-3 activity and the number of apoptotic cells. Conclusions: SIS3 ameliorated fibrosis, apoptosis, and inflammation through inhibition of TGF-?/Smad3 signaling in UUO mouse kidneys.