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Delphinidin induced protective autophagy via mTOR pathway suppression and AMPK
pathway activation in HER-2 positive breast cancer cells
#MMPMID29587684
Chen J
; Zhu Y
; Zhang W
; Peng X
; Zhou J
; Li F
; Han B
; Liu X
; Ou Y
; Yu X
BMC Cancer
2018[Mar]; 18
(1
): 342
PMID29587684
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BACKGROUND: We have previously demonstrated the anticancer effect of
anthocyanins. In this study, we explored the biological activities of
delphinidin, the most common of the anthocyanidin monomers, that were related to
autophagy in HER-2 positive breast cancer MDA-MB-453 and BT474 cells. METHODS:
The effects of various doses of delphinidin on the proliferation and apoptosis of
MDA-MB-453 and BT474 cells were analysed. Autophagy was identified as a critical
factor that influenced chemotherapy, and the autophagic mechanism in
delphinidin-treated cells was investigated. The autophagy inhibitors, 3-MA and
BA1, were used to analyse the effects of autophagy inhibition. RESULTS:
Delphinidin inhibited proliferation, promoted apoptosis, and induced autophagy in
MDA-MB-453 and BT474 cells in a dose-dependent manner. The inhibition of
autophagy enhanced the delphinidin-induced apoptosis and antiproliferative effect
in both HER-2 positive breast cancer cells. In addition, delphinidin induced
autophagy via suppression of the mTOR signalling pathway and activation of the
AMPK signalling pathway in HER-2 positive breast cancer cells. CONCLUSIONS:
Collectively, the results showed that delphinidin induced apoptosis and autophagy
in HER-2 positive breast cancer cells and that autophagy was induced via the mTOR
and AMPK signalling pathways. The suppression of autophagy promoted the
anticancer effects of delphinidin.
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