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Switch of the ovarian cancer cell to a calcifying phenotype in the calcification
of ovarian cancer
#MMPMID29581780
Wen J
; Zhao Z
; Huang L
; Li L
; Li J
; Zeng Y
; Wu J
; Miao Y
J Cancer
2018[]; 9
(6
): 1006-1016
PMID29581780
show ga
OBJECTIVE: The main aim of this study was to study swith of the ovarian cancer
cell to a calcifying phenotype in the formation of calcification in ovarian
cancer, and to offer some help for ovarian cancer's diagnosis and differentiation
therapy. METHODS: The mineralization of ovarian cancer cell lines SKOV3 was
induced via calcification medium for 21 d in vitro. Alizarin red staining, von
kossa staining, calcein fluorescence staining and ALP activity detection were
used to identify mineralization in calcification model of ovarian cancer. Also,
the changes of ultrastructure and the mineralization biomarkers after the
induction of calcification medium were investigated by transmission electron
microscopy and western blot, respectively. The SKOV3 cells migration behavior
after the induction of calcification medium was evaluated by using transwell
assay and scratch wound. Finally, mineralization biomarkers were verified in 40
cases of calcified ovarian cancer specimens and matched 40 non-calcified ovarian
cancer tissues. RESULTS: Classical calcium salt detection methods confirmed that
the culture of SKOV3 cells in calcification medium was an appropriate ovarian
cancer calcification model in vitro. Transmission electron microscopy and western
blot revealed respectively the presence of cells with morphological
characteristics of osteoblasts and the upregulation of mineralization biomarkers
expression in treatment group. Transwell assay and scratch wound showed the
decreased SKOV3 cell migration in treatment group. In specimens, the
calcification occurred predominantly in well-differentiated carcinomas and the
expression of the BMP2 and OPN elevated in calcified group. CONCLUSION: Our study
showed that the switch of the ovarian cancer cell to a calcifying phenotype in
the formation of calcification in ovarian cancer. The calcified phenotypic
transformation may inform the new prospective in ovarian cancer therapy.
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