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10.3892/mmr.2017.7422 http://scihub22266oqcxt.onion/10.3892/mmr.2017.7422 C5865827!5865827 !28901424     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28901424 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Med+Rep 2017 ; 16 (5 ): 6729-6735 Nephropedia Template TP {{tp|p=28901424 |t=2017. Tumor suppressor microRNA?613 inhibits glioma cell proliferation, invasion and angiogenesis by targeting vascular endothelial growth factor A |pdf=|usr=}}{{28901424 }} gab.com Text Tumor suppressor microRNA 613 inhibits glioma cell proliferation, invasion and angiogenesis by targeting vascular endothelial growth factor A JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=28901424 #FOAvip MicroRNAs (miRNAs) are small non?coding RNAs which can serve as oncogenes or tumor suppressors in glioma. The present study aimed to investigate the expression of miR?613 in glioma. Reverse transcription?quantitative polymerase chain reaction (RT?qPCR) was used to detect miR?613 in glioma cells and tissues and the relationship between miR?613 and vascular endothelial growth factor (VEGF) A was assessed using a luciferase reporter assay. In addition, glioma cells were transfected with miR?613 mimics and the mRNA and protein expression of VEGFA was detected using RT?qPCR and western blot analysis, respectively. The proliferative, invasive and tube formation capabilities of transfected cells were also assessed in vitro. Furthermore, a nude mouse tumor xenograft model was used to investigate the effects of miR?613 on tumor growth in vivo. The results of the present study demonstrated that the expression of miR?613 was decreased in glioma cell lines, and was associated with the grade of glioma. Ectopic expression of miR?613 markedly suppressed glioma cell proliferation and angiogenesis. Furthermore, the upregulation of miR?613 inhibited tumor angiogenesis and tumor growth in xenografted nude mice in vivo. VEGFA was demonstrated as a direct target of miR?613, as detected by western blot and luciferase reporter assays, and mediated miR?613 induced glioma cell proliferation and angiogenesis inhibition. Twit Text FOAVip Tumor suppressor microRNA 613 inhibits glioma cell proliferation, invasion and angiogenesis by targeting vascular endothelial growth factor A ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=28901424 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28901424 #FOAvip English Wikipedia <ref>{{Cite pmid|28901424 }}</ref> Tumor suppressor microRNA?613 inhibits glioma cell proliferation, invasion and angiogenesis by targeting vascular endothelial growth factor A #MMPMID28901424 Yu X ; Wang W Mol Med Rep 2017[Nov]; 16 (5 ): 6729-6735 PMID28901424 show ga MicroRNAs (miRNAs) are small non?coding RNAs which can serve as oncogenes or tumor suppressors in glioma. The present study aimed to investigate the expression of miR?613 in glioma. Reverse transcription?quantitative polymerase chain reaction (RT?qPCR) was used to detect miR?613 in glioma cells and tissues and the relationship between miR?613 and vascular endothelial growth factor (VEGF) A was assessed using a luciferase reporter assay. In addition, glioma cells were transfected with miR?613 mimics and the mRNA and protein expression of VEGFA was detected using RT?qPCR and western blot analysis, respectively. The proliferative, invasive and tube formation capabilities of transfected cells were also assessed in vitro. Furthermore, a nude mouse tumor xenograft model was used to investigate the effects of miR?613 on tumor growth in vivo. The results of the present study demonstrated that the expression of miR?613 was decreased in glioma cell lines, and was associated with the grade of glioma. Ectopic expression of miR?613 markedly suppressed glioma cell proliferation and angiogenesis. Furthermore, the upregulation of miR?613 inhibited tumor angiogenesis and tumor growth in xenografted nude mice in vivo. VEGFA was demonstrated as a direct target of miR?613, as detected by western blot and luciferase reporter assays, and mediated miR?613 induced glioma cell proliferation and angiogenesis inhibition. |3' Untranslated Regions [MESH] |Animals [MESH] |Antagomirs/metabolism [MESH] |Base Sequence [MESH] |Brain Neoplasms/blood supply/drug therapy/genetics/*pathology [MESH] |Cell Line, Tumor [MESH] |Cell Movement [MESH] |Cell Proliferation [MESH] |Glioma/blood supply/drug therapy/genetics/*pathology [MESH] |Humans [MESH] |Mice [MESH] |Mice, Nude [MESH] |MicroRNAs/antagonists & inhibitors/*metabolism/therapeutic use [MESH] |Neoplasm Staging [MESH] |Neovascularization, Pathologic/prevention & control [MESH] |Sequence Alignment [MESH] |Transplantation, Heterologous [MESH]Tumor suppressor microRNA?613 inhibits glioma cell proliferation, inva(epmc).pdf DeepDyve Pubget Overpricing