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Alterations in the long non?coding RNA transcriptome in mesangial cells treated
with aldosterone in vitro
#MMPMID28849035
Qu G
; Shi H
; Wang B
; Li S
; Zhang A
; Gan W
Mol Med Rep
2017[Nov]; 16
(5
): 6004-6012
PMID28849035
show ga
Clinical and experimental reports indicate that aldosterone (ALD) contributes to
the progression of renal failure independent of its hemodynamic effects. However,
the mechanisms remain to be completely elucidated. The aim of the present study
was to investigate the alterations of long non?coding RNA (lncRNA) in mesangial
cells (MCs) treated with ALD. The present study used MCs treated with 10?6 M ALD
as experimental cells. Microarray techniques performed by Agilent Technologies
were used to identify the profiles of differentially expressed lncRNAs between
the ALD group and the control group. Pathway and gene ontology analysis were
applied to determine the roles of the differentially expressed lncRNAs. Reverse
transcription quantitative polymerase chain reaction (RT?qPCR) was used to
quantify the differentially expressed lncRNAs. A total of 8,459 lncRNA and 13,214
mRNAs with differential expression between MCs treated with and without ALD were
identified. The expression of lncRNAs was confirmed by RT?qPCR and the results
were consistent with the lncRNA array. The biological functions of lncRNAs are
associated with responding to external stimuli, positive regulation of biological
and apoptotic processes, cell division, mitosis and nuclear division. The
pathways include cell cycle and peroxisome proliferator?activated receptor
signaling pathways. The present study revealed distinct sets of lncRNA expressed
in MCs treated with ALD, suggesting that this class of transcripts may be
involved in the pathogenesis of chronic kidney diseases.
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