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10.1126/science.aal2066 http://scihub22266oqcxt.onion/10.1126/science.aal2066 C5865750!5865750!28619718     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2017 ; 356 (6345): 1397-401 Nephropedia Template TP {{tp|p=28619718|t=2017. Click chemistry enables preclinical evaluation of targeted epigenetic therapies |pdf=|usr=}}{{28619718}} gab.com Text Click chemistry enables preclinical evaluation of targeted epigenetic therapies JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=28619718 #FOAvip The success of new therapies hinges on our ability to understand their molecular and cellular mechanisms of action. We modified BET bromodomain inhibitors, an epigenetic-based therapy, to create functionally conserved compounds that are amenable to click chemistry and can be used as molecular probes in vitro and in vivo. We used click proteomics and click sequencing to explore the gene regulatory function of BRD4 (bromodomain containing protein 4) and the transcriptional changes induced by BET inhibitors. In our studies of mouse models of acute leukemia, we used high-resolution microscopy and flow cytometry to highlight the heterogeneity of drug activity within tumor cells located in different tissue compartments. We also demonstrate the differential distribution and effects of BET inhibitors in normal and malignant cells in vivo. This study provides a potential framework for the preclinical assessment of a wide range of drugs. Twit Text FOAVip Click chemistry enables preclinical evaluation of targeted epigenetic therapies ????Science http://www.kidney.de/pget.php?&tw=1&pmid=28619718 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28619718 #FOAvip English Wikipedia <ref>{{Cite pmid|28619718}}</ref> Click chemistry enables preclinical evaluation of targeted epigenetic therapies #MMPMID28619718 Tyler DS; Vappiani J; Cañeque T; Lam EYN; Ward A; Gilan O; Chan YC; Hienzsch A; Rutkowska A; Werner T; Wagner AJ; Lugo D; Gregory R; Molina CR; Garton N; Wellaway CR; Jackson S; MacPherson L; Figueiredo M; Stolzenburg S; Bell CC; House C; Dawson SJ; Hawkins ED; Drewes G; Prinjha RK; Rodriguez R; Grandi P; Dawson MA Science 2017[Jun]; 356 (6345): 1397-401 PMID28619718show ga The success of new therapies hinges on our ability to understand their molecular and cellular mechanisms of action. We modified BET bromodomain inhibitors, an epigenetic-based therapy, to create functionally conserved compounds that are amenable to click chemistry and can be used as molecular probes in vitro and in vivo. We used click proteomics and click sequencing to explore the gene regulatory function of BRD4 (bromodomain containing protein 4) and the transcriptional changes induced by BET inhibitors. In our studies of mouse models of acute leukemia, we used high-resolution microscopy and flow cytometry to highlight the heterogeneity of drug activity within tumor cells located in different tissue compartments. We also demonstrate the differential distribution and effects of BET inhibitors in normal and malignant cells in vivo. This study provides a potential framework for the preclinical assessment of a wide range of drugs. äClick chemistry enables preclinical evaluation of targeted epigenetic (epmc).pdf DeepDyve Pubget Overpricing