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10.1038/lsa.2017.95 http://scihub22266oqcxt.onion/10.1038/lsa.2017.95 C5863928!5863928!29576887     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Light+Sci+Appl 2017 ; 6 (11): e17095- Nephropedia Template TP {{tp|p=29576887|t=2017. Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser |pdf=|usr=}}{{29576887}} gab.com Text Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser JO: Light Sci Appl http://www.kidney.de/pget.php?&tw=1&pmid=29576887 #FOAvip Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of excitable fluorophores without the need for wavelength tuning. However, most currently available dual output laser sources rely on the costly and complicated optical parametric generation approach. In this report, we detail a relatively simple and low cost diamond Raman laser pumped by a ytterbium fiber amplifier emitting at 1055?nm, which generates a first Stokes emission centered at 1240?nm with a pulse width of 100?fs. The two excitation wavelengths of 1055 and 1240?nm, along with the effective two-color excitation wavelength of 1140?nm, provide an almost complete coverage of fluorophores excitable within the range of 1000?1300?nm. When compared with 1055?nm excitation, two-color excitation at 1140?nm offers a 90% increase in signal for many far-red emitting fluorescent proteins (for example, tdKatushka2). We demonstrate multicolor imaging of tdKatushka2 and Hoechst 33342 via simultaneous two-color two-photon, and two-color three-photon microscopy in engineered 3D multicellular spheroids. We further discuss potential benefits and applications for two-color three-photon excitation. In addition, we show that this laser system is capable of in vivo imaging in mouse cortex to nearly 1?mm in depth with two-color excitation. Twit Text FOAVip Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser ????Light Sci Appl http://www.kidney.de/pget.php?&tw=1&pmid=29576887 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29576887 #FOAvip English Wikipedia <ref>{{Cite pmid|29576887}}</ref> Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser #MMPMID29576887 Perillo EP; Jarrett JW; Liu YL; Hassan A; Fernée DC; Goldak JR; Bonteanu A; Spence DJ; Yeh HC; Dunn AK Light Sci Appl 2017[Nov]; 6 (11): e17095- PMID29576887show ga Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of excitable fluorophores without the need for wavelength tuning. However, most currently available dual output laser sources rely on the costly and complicated optical parametric generation approach. In this report, we detail a relatively simple and low cost diamond Raman laser pumped by a ytterbium fiber amplifier emitting at 1055?nm, which generates a first Stokes emission centered at 1240?nm with a pulse width of 100?fs. The two excitation wavelengths of 1055 and 1240?nm, along with the effective two-color excitation wavelength of 1140?nm, provide an almost complete coverage of fluorophores excitable within the range of 1000?1300?nm. When compared with 1055?nm excitation, two-color excitation at 1140?nm offers a 90% increase in signal for many far-red emitting fluorescent proteins (for example, tdKatushka2). We demonstrate multicolor imaging of tdKatushka2 and Hoechst 33342 via simultaneous two-color two-photon, and two-color three-photon microscopy in engineered 3D multicellular spheroids. We further discuss potential benefits and applications for two-color three-photon excitation. In addition, we show that this laser system is capable of in vivo imaging in mouse cortex to nearly 1?mm in depth with two-color excitation. äTwo-color multiphoton in vivo imaging with a femtosecond diamond Raman(epmc).pdf DeepDyve Pubget Overpricing