Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1186/s13059-018-1413-5 http://scihub22266oqcxt.onion/10.1186/s13059-018-1413-5 C5863892!5863892!29566733     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2018 ; 19 (ä): ä Nephropedia Template TP {{tp|p=29566733|t=2018. Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging |pdf=|usr=}}{{29566733}} gab.com Text Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=29566733 #FOAvip CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dCas9/gRNA genome labeling systems. Surprisingly, we discover that in the gRNA-labeling strategies, accumulation of tagged gRNA transcripts leads to non-specific labeling foci. Furthermore, we develop novel bimolecular fluorescence complementation (BIFC) methods that combine the advantages of both dCas9-labeling and gRNA-labeling strategies. The BIFC-dCas9/gRNA methods demonstrate high signal-to-noise ratios and have no non-specific foci. Twit Text FOAVip Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=29566733 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29566733 #FOAvip English Wikipedia <ref>{{Cite pmid|29566733}}</ref> Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging #MMPMID29566733 Hong Y; Lu G; Duan J; Liu W; Zhang Y Genome Biol 2018[]; 19 (ä): ä PMID29566733show ga CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dCas9/gRNA genome labeling systems. Surprisingly, we discover that in the gRNA-labeling strategies, accumulation of tagged gRNA transcripts leads to non-specific labeling foci. Furthermore, we develop novel bimolecular fluorescence complementation (BIFC) methods that combine the advantages of both dCas9-labeling and gRNA-labeling strategies. The BIFC-dCas9/gRNA methods demonstrate high signal-to-noise ratios and have no non-specific foci. äComparison and optimization of CRISPR/dCas9/gRNA genome-labeling syste(epmc).pdf DeepDyve Pubget Overpricing