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10.1093/nar/gkx1222 http://scihub22266oqcxt.onion/10.1093/nar/gkx1222 C5861443!5861443!29237052     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2018 ; 46 (5): e25 Nephropedia Template TP {{tp|p=29237052|t=2018. Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing |pdf=|usr=}}{{29237052}} gab.com Text Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=29237052 #FOAvip Precise investigation and manipulation of dynamic biological processes often requires molecular modulation in a controlled inducible manner. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) has emerged as a versatile tool for targeted gene editing and transcriptional programming. Here, we designed and vigorously optimized a series of Hybrid drug Inducible CRISPR/Cas9 Technologies (HIT) for transcriptional activation by grafting a mutated human estrogen receptor (ERT2) to multiple CRISPR/Cas9 systems, which renders them 4-hydroxytamoxifen (4-OHT) inducible for the access of genome. Further, extra functionality of simultaneous genome editing was achieved with one device we named HIT2. Optimized terminal devices herein delivered advantageous performances in comparison with several existing designs. They exerted selective, titratable, rapid and reversible response to drug induction. In addition, these designs were successfully adapted to an orthogonal Cas9. HIT systems developed in this study can be applied for controlled modulation of potentially any genomic loci in multiple modes. Twit Text FOAVip Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=29237052 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29237052 #FOAvip English Wikipedia <ref>{{Cite pmid|29237052}}</ref> Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing #MMPMID29237052 Lu J; Zhao C; Zhao Y; Zhang J; Zhang Y; Chen L; Han Q; Ying Y; Peng S; Ai R; Wang Y Nucleic Acids Res 2018[Mar]; 46 (5): e25 PMID29237052show ga Precise investigation and manipulation of dynamic biological processes often requires molecular modulation in a controlled inducible manner. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) has emerged as a versatile tool for targeted gene editing and transcriptional programming. Here, we designed and vigorously optimized a series of Hybrid drug Inducible CRISPR/Cas9 Technologies (HIT) for transcriptional activation by grafting a mutated human estrogen receptor (ERT2) to multiple CRISPR/Cas9 systems, which renders them 4-hydroxytamoxifen (4-OHT) inducible for the access of genome. Further, extra functionality of simultaneous genome editing was achieved with one device we named HIT2. Optimized terminal devices herein delivered advantageous performances in comparison with several existing designs. They exerted selective, titratable, rapid and reversible response to drug induction. In addition, these designs were successfully adapted to an orthogonal Cas9. HIT systems developed in this study can be applied for controlled modulation of potentially any genomic loci in multiple modes. äMultimode drug inducible CRISPR/Cas9 devices for transcriptional activ(epmc).pdf DeepDyve Pubget Overpricing