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10.1371/journal.pbio.2003067 http://scihub22266oqcxt.onion/10.1371/journal.pbio.2003067 C5860796!5860796!29505568     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Biol 2018 ; 16 (3): ä Nephropedia Template TP {{tp|p=29505568|t=2018. Spliced integrated retrotransposed element (SpIRE) formation in the human genome |pdf=|usr=}}{{29505568}} gab.com Text Spliced integrated retrotransposed element (SpIRE) formation in the human genome JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=29505568 #FOAvip Human Long interspersed element-1 (L1) retrotransposons contain an internal RNA polymerase II promoter within their 5? untranslated region (UTR) and encode two proteins, (ORF1p and ORF2p) required for their mobilization (i.e., retrotransposition). The evolutionary success of L1 relies on the continuous retrotransposition of full-length L1 mRNAs. Previous studies identified functional splice donor (SD), splice acceptor (SA), and polyadenylation sequences in L1 mRNA and provided evidence that a small number of spliced L1 mRNAs retrotransposed in the human genome. Here, we demonstrate that the retrotransposition of intra-5?UTR or 5?UTR/ORF1 spliced L1 mRNAs leads to the generation of spliced integrated retrotransposed elements (SpIREs). We identified a new intra-5?UTR SpIRE that is ten times more abundant than previously identified SpIREs. Functional analyses demonstrated that both intra-5?UTR and 5?UTR/ORF1 SpIREs lack Cis-acting transcription factor binding sites and exhibit reduced promoter activity. The 5?UTR/ORF1 SpIREs also produce nonfunctional ORF1p variants. Finally, we demonstrate that sequence changes within the L1 5?UTR over evolutionary time, which permitted L1 to evade the repressive effects of a host protein, can lead to the generation of new L1 splicing events, which, upon retrotransposition, generates a new SpIRE subfamily. We conclude that splicing inhibits L1 retrotransposition, SpIREs generally represent evolutionary ?dead-ends? in the L1 retrotransposition process, mutations within the L1 5?UTR alter L1 splicing dynamics, and that retrotransposition of the resultant spliced transcripts can generate interindividual genomic variation. Twit Text FOAVip Spliced integrated retrotransposed element (SpIRE) formation in the human genome ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=29505568 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29505568 #FOAvip English Wikipedia <ref>{{Cite pmid|29505568}}</ref> Spliced integrated retrotransposed element (SpIRE) formation in the human genome #MMPMID29505568 Larson PA; Moldovan JB; Jasti N; Kidd JM; Beck CR; Moran JV PLoS Biol 2018[Mar]; 16 (3): ä PMID29505568show ga Human Long interspersed element-1 (L1) retrotransposons contain an internal RNA polymerase II promoter within their 5? untranslated region (UTR) and encode two proteins, (ORF1p and ORF2p) required for their mobilization (i.e., retrotransposition). The evolutionary success of L1 relies on the continuous retrotransposition of full-length L1 mRNAs. Previous studies identified functional splice donor (SD), splice acceptor (SA), and polyadenylation sequences in L1 mRNA and provided evidence that a small number of spliced L1 mRNAs retrotransposed in the human genome. Here, we demonstrate that the retrotransposition of intra-5?UTR or 5?UTR/ORF1 spliced L1 mRNAs leads to the generation of spliced integrated retrotransposed elements (SpIREs). We identified a new intra-5?UTR SpIRE that is ten times more abundant than previously identified SpIREs. Functional analyses demonstrated that both intra-5?UTR and 5?UTR/ORF1 SpIREs lack Cis-acting transcription factor binding sites and exhibit reduced promoter activity. The 5?UTR/ORF1 SpIREs also produce nonfunctional ORF1p variants. Finally, we demonstrate that sequence changes within the L1 5?UTR over evolutionary time, which permitted L1 to evade the repressive effects of a host protein, can lead to the generation of new L1 splicing events, which, upon retrotransposition, generates a new SpIRE subfamily. We conclude that splicing inhibits L1 retrotransposition, SpIREs generally represent evolutionary ?dead-ends? in the L1 retrotransposition process, mutations within the L1 5?UTR alter L1 splicing dynamics, and that retrotransposition of the resultant spliced transcripts can generate interindividual genomic variation. äSpliced integrated retrotransposed element (SpIRE) formation in the hu(epmc).pdf DeepDyve Pubget Overpricing