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10.1016/j.abb.2018.01.017 http://scihub22266oqcxt.onion/10.1016/j.abb.2018.01.017 C5856089!5856089!29410086     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arch+Biochem+Biophys 2018 ; 642 (ä): 46-51 Nephropedia Template TP {{tp|p=29410086|t=2018. Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems |pdf=|usr=}}{{29410086}} gab.com Text Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems JO: Arch Biochem Biophys http://www.kidney.de/pget.php?&tw=1&pmid=29410086 #FOAvip Apaf-1 is a cytosolic multi-domain protein in the apoptosis regulatory network. When cytochrome c releases from mitochondria; it binds to WD-40 repeats of Apaf-1 molecule and induces oligomerization of Apaf-1. Here in, a split luciferase assay was used to compare apoptosome formation in cell-free and cell-based systems. This assay uses Apaf-1 tagged with either N-terminal fragment or C-terminal fragment of P. pyralis luciferase. In cell based-system, the apoptosome formation is induced inside the cells which express Apaf-1 tagged with complementary fragments of luciferase while in cell-free system, the apoptosome formation is induced in extracts of the cells. In cell-free system, cytochrome c dependent luciferase activity was observed with full length Apaf-1. However, luciferase activity due to apoptosome formation was much higher in cell based system compared to cell-free system. The truncated Apaf-1 which lacks WD-40 repeats (?Apaf-1) interacted with endogenous Apaf-1 in a different fashion compared to native form as confirmed by different retention time of eluate in gel filtration and binding to affinity column. The interactions between endogenous Apaf-1 and ?Apaf-1 is stronger than its interaction with native exogenous Apaf-1 as indicated by dominant negative effect of ?Apaf-1 on caspase-3 processing. Twit Text FOAVip Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems ????Arch Biochem Biophys http://www.kidney.de/pget.php?&tw=1&pmid=29410086 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29410086 #FOAvip English Wikipedia <ref>{{Cite pmid|29410086}}</ref> Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems #MMPMID29410086 Noori AR; Hosseini ES; Nikkhah M; Hosseinkhani S Arch Biochem Biophys 2018[Mar]; 642 (ä): 46-51 PMID29410086show ga Apaf-1 is a cytosolic multi-domain protein in the apoptosis regulatory network. When cytochrome c releases from mitochondria; it binds to WD-40 repeats of Apaf-1 molecule and induces oligomerization of Apaf-1. Here in, a split luciferase assay was used to compare apoptosome formation in cell-free and cell-based systems. This assay uses Apaf-1 tagged with either N-terminal fragment or C-terminal fragment of P. pyralis luciferase. In cell based-system, the apoptosome formation is induced inside the cells which express Apaf-1 tagged with complementary fragments of luciferase while in cell-free system, the apoptosome formation is induced in extracts of the cells. In cell-free system, cytochrome c dependent luciferase activity was observed with full length Apaf-1. However, luciferase activity due to apoptosome formation was much higher in cell based system compared to cell-free system. The truncated Apaf-1 which lacks WD-40 repeats (?Apaf-1) interacted with endogenous Apaf-1 in a different fashion compared to native form as confirmed by different retention time of eluate in gel filtration and binding to affinity column. The interactions between endogenous Apaf-1 and ?Apaf-1 is stronger than its interaction with native exogenous Apaf-1 as indicated by dominant negative effect of ?Apaf-1 on caspase-3 processing. äApoptosome formation upon overexpression of native and truncated Apaf-(epmc).pdf DeepDyve Pubget Overpricing