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10.3390/ijms19020638

http://scihub22266oqcxt.onion/10.3390/ijms19020638
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C5855860!5855860 !29495267
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suck abstract from ncbi


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pmid29495267
      Int+J+Mol+Sci 2018 ; 19 (2 ): ä
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  • Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation #MMPMID29495267
  • Huang FC ; Fu BJ ; Liu YT ; Chang YR ; Chi SF ; Chien PR ; Huang SC ; Hwang HH
  • Int J Mol Sci 2018[Feb]; 19 (2 ): ä PMID29495267 show ga
  • Agrobacterium tumefaciens can genetically transform various eukaryotic cells because of the presence of a resident tumor-inducing (Ti) plasmid. During infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is transferred from bacteria into plant cells and causes plant cells to abnormally synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and several virulence (Vir) proteins are secreted through a type IV secretion system (T4SS) composed of T-pilus and a transmembrane protein complex. Three members of Arabidopsis reticulon-like B (RTNLB) proteins, RTNLB1, 2, and 4, interact with VirB2, the major component of T-pilus. Here, we have identified that other RTNLB proteins, RTNLB3 and 8, interact with VirB2 in vitro. Root-based A. tumefaciens transformation assays with Arabidopsis rtnlb3, or rtnlb5-10 single mutants showed that the rtnlb8 mutant was resistant to A. tumefaciens infection. In addition, rtnlb3 and rtnlb8 mutants showed reduced transient transformation efficiency in seedlings. RTNLB3- or 8 overexpression transgenic plants showed increased susceptibility to A. tumefaciens and Pseudomonas syringae infection. RTNLB1-4 and 8 transcript levels differed in roots, rosette leaves, cauline leaves, inflorescence, flowers, and siliques of wild-type plants. Taken together, RTNLB3 and 8 may participate in A. tumefaciens infection but may have different roles in plants.
  • |*Transformation, Genetic [MESH]
  • |Agrobacterium/*physiology [MESH]
  • |Arabidopsis Proteins/*genetics/metabolism [MESH]
  • |Arabidopsis/*genetics/*microbiology [MESH]
  • |DNA, Bacterial/genetics [MESH]
  • |Disease Susceptibility [MESH]
  • |Gene Expression [MESH]
  • |Gene Expression Regulation, Plant [MESH]
  • |Host-Pathogen Interactions [MESH]
  • |Membrane Proteins/*genetics/metabolism [MESH]
  • |Mutation [MESH]
  • |Organ Specificity [MESH]
  • |Plant Diseases/genetics/microbiology [MESH]
  • |Plants, Genetically Modified [MESH]
  • |Protein Binding [MESH]
  • |Recombinant Fusion Proteins [MESH]


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