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2018 ; 19
(2
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in
Agrobacterium-Mediated Plant Transformation
#MMPMID29495267
Huang FC
; Fu BJ
; Liu YT
; Chang YR
; Chi SF
; Chien PR
; Huang SC
; Hwang HH
Int J Mol Sci
2018[Feb]; 19
(2
): ä PMID29495267
show ga
Agrobacterium tumefaciens can genetically transform various eukaryotic cells
because of the presence of a resident tumor-inducing (Ti) plasmid. During
infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is
transferred from bacteria into plant cells and causes plant cells to abnormally
synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and
several virulence (Vir) proteins are secreted through a type IV secretion system
(T4SS) composed of T-pilus and a transmembrane protein complex. Three members of
Arabidopsis reticulon-like B (RTNLB) proteins, RTNLB1, 2, and 4, interact with
VirB2, the major component of T-pilus. Here, we have identified that other RTNLB
proteins, RTNLB3 and 8, interact with VirB2 in vitro. Root-based A. tumefaciens
transformation assays with Arabidopsis rtnlb3, or rtnlb5-10 single mutants showed
that the rtnlb8 mutant was resistant to A. tumefaciens infection. In addition,
rtnlb3 and rtnlb8 mutants showed reduced transient transformation efficiency in
seedlings. RTNLB3- or 8 overexpression transgenic plants showed increased
susceptibility to A. tumefaciens and Pseudomonas syringae infection. RTNLB1-4 and
8 transcript levels differed in roots, rosette leaves, cauline leaves,
inflorescence, flowers, and siliques of wild-type plants. Taken together, RTNLB3
and 8 may participate in A. tumefaciens infection but may have different roles in
plants.