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2018 ; 9
(2
): 529-542
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Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2
tumor biomarker in human plasma
#MMPMID29552391
Rizzo R
; Alvaro M
; Danz N
; Napione L
; Descrovi E
; Schmieder S
; Sinibaldi A
; Rana S
; Chandrawati R
; Munzert P
; Schubert T
; Maillart E
; Anopchenko A
; Rivolo P
; Mascioletti A
; Förster E
; Sonntag F
; Stevens MM
; Bussolino F
; Michelotti F
Biomed Opt Express
2018[Feb]; 9
(2
): 529-542
PMID29552391
show ga
Quantitative detection of angiogenic biomarkers provides a powerful tool to
diagnose cancers in early stages and to follow its progression during therapy.
Conventional tests require trained personnel, dedicated laboratory equipment and
are generally time-consuming. Herein, we propose our developed biosensing
platform as a useful tool for a rapid determination of Angiopoietin-2 biomarker
directly from patient plasma within 30 minutes, without any sample preparation or
dilution. Bloch surface waves supported by one dimensional photonic crystal are
exploited to enhance and redirect the fluorescence arising from a sandwich
immunoassay that involves Angiopoietin-2. The sensing units consist of disposable
and low-cost plastic biochips coated with the photonic crystal. The biosensing
platform is demonstrated to detect Angiopoietin-2 in plasma samples at the
clinically relevant concentration of 6 ng/mL, with an estimated limit of
detection of approximately 1 ng/mL. This is the first Bloch surface wave based
assay capable of detecting relevant concentrations of an angiogenic factor in
plasma samples. The results obtained by the developed biosensing platform are in
close agreement with enzyme-linked immunosorbent assays, demonstrating a good
accuracy, and their repeatability showed acceptable relative variations.
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