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10.1364/BOE.9.000423 http://scihub22266oqcxt.onion/10.1364/BOE.9.000423 C5854048!5854048!29552383     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Opt+Express 2018 ; 9 (2): 423-36 Nephropedia Template TP {{tp|p=29552383|t=2018. Imaging transparent intact cardiac tissue with single-cell resolution |pdf=|usr=}}{{29552383}} gab.com Text Imaging transparent intact cardiac tissue with single-cell resolution JO: Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=29552383 #FOAvip We developed a new method, SUT (Scheme Update on tissue Transparency), to view cardiac microstructures and unveil the molecular changes underlying cardiac diseases. SUT is an effective method to clear whole-hearts from different species. Over the course of 4 - 6 days we obtained transparent whole-layer left ventricular tissues from mice with only an approximate 1% protein loss. In addition, EAL (Electrophoretic Antibody Labeling) was used to achieve fast antibody labeling by electric force, which significantly reduced antibody incubation time from days to hours. SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue. We also observed changes in the number and phenotypes of fibroblasts during post-myocardial infarction in a stereoscopic pattern. We believe that our technique opens a new avenue to explore the mechanisms underlying cardiac diseases. Twit Text FOAVip Imaging transparent intact cardiac tissue with single-cell resolution ????Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=29552383 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29552383 #FOAvip English Wikipedia <ref>{{Cite pmid|29552383}}</ref> Imaging transparent intact cardiac tissue with single-cell resolution #MMPMID29552383 Wang Z; Zhang J; Fan G; Zhao H; Wang X; Zhang J; Zhang P; Wang W Biomed Opt Express 2018[Feb]; 9 (2): 423-36 PMID29552383show ga We developed a new method, SUT (Scheme Update on tissue Transparency), to view cardiac microstructures and unveil the molecular changes underlying cardiac diseases. SUT is an effective method to clear whole-hearts from different species. Over the course of 4 - 6 days we obtained transparent whole-layer left ventricular tissues from mice with only an approximate 1% protein loss. In addition, EAL (Electrophoretic Antibody Labeling) was used to achieve fast antibody labeling by electric force, which significantly reduced antibody incubation time from days to hours. SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue. We also observed changes in the number and phenotypes of fibroblasts during post-myocardial infarction in a stereoscopic pattern. We believe that our technique opens a new avenue to explore the mechanisms underlying cardiac diseases. äImaging transparent intact cardiac tissue with single-cell resolution (epmc).pdf DeepDyve Pubget Overpricing