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10.1093/jxb/erx183 http://scihub22266oqcxt.onion/10.1093/jxb/erx183 C5853561!5853561 !28586477     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28586477 &cmd=llinks): Failed to open stream: HTTP request failed! 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Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases |pdf=|usr=}}{{28586477 }} gab.com Text Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases JO: J Exp Bot http://www.kidney.de/pget.php?&tw=1&pmid=28586477 #FOAvip Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, analysis of protein-metabolite and protein-protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1-NDPK3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside diphosphates (NDPs) and triphosphates (NTPs). However, other functions have been reported, which probably depend on both the proteins and small molecules specifically interacting with the NDPK. Using our approach we identified 23, 17, and 8 novel protein partners of NDPK1, NDPK2, and NDPK3, respectively, with nucleotide-dependent proteins such as actin and adenosine kinase 2 being enriched. Particularly interesting, however, was the co-elution of glutathione S-transferases (GSTs) and reduced glutathione (GSH) with the affinity-purified NDPK1 complexes. Following up on this finding, we could demonstrate that NDPK1 undergoes glutathionylation, opening a new paradigm of NDPK regulation in plants. The described results extend our knowledge of NDPKs, the key enzymes regulating NDP/NTP homeostasis. Twit Text FOAVip Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases ????J Exp Bot http://www.kidney.de/pget.php?&tw=1&pmid=28586477 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28586477 #FOAvip English Wikipedia <ref>{{Cite pmid|28586477 }}</ref> Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases #MMPMID28586477 Luzarowski M ; Kosmacz M ; Sokolowska E ; Jasinska W ; Willmitzer L ; Veyel D ; Skirycz A J Exp Bot 2017[Jun]; 68 (13 ): 3487-3499 PMID28586477 show ga Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, analysis of protein-metabolite and protein-protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1-NDPK3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside diphosphates (NDPs) and triphosphates (NTPs). However, other functions have been reported, which probably depend on both the proteins and small molecules specifically interacting with the NDPK. Using our approach we identified 23, 17, and 8 novel protein partners of NDPK1, NDPK2, and NDPK3, respectively, with nucleotide-dependent proteins such as actin and adenosine kinase 2 being enriched. Particularly interesting, however, was the co-elution of glutathione S-transferases (GSTs) and reduced glutathione (GSH) with the affinity-purified NDPK1 complexes. Following up on this finding, we could demonstrate that NDPK1 undergoes glutathionylation, opening a new paradigm of NDPK regulation in plants. The described results extend our knowledge of NDPKs, the key enzymes regulating NDP/NTP homeostasis. |*Chromatography, Affinity [MESH] |*Metabolomics [MESH] |*Proteomics [MESH] |Arabidopsis/*genetics [MESH] |Nucleoside-Diphosphate Kinase/*genetics/metabolism [MESH]Affinity purification with metabolomic and proteomic analysis unravels(epmc).pdf DeepDyve Pubget Overpricing