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10.1093/infdis/jix171 http://scihub22266oqcxt.onion/10.1093/infdis/jix171 C5853400!5853400 !28398522     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28398522 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Infect+Dis 2017 ; 216 (2 ): 162-171 Nephropedia Template TP {{tp|p=28398522 |t=2017. Zika Virus Infection of the Human Glomerular Cells: Implications for Viral Reservoirs and Renal Pathogenesis |pdf=|usr=}}{{28398522 }} gab.com Text Zika Virus Infection of the Human Glomerular Cells: Implications for Viral Reservoirs and Renal Pathogenesis JO: J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=28398522 #FOAvip BACKGROUND: Zika virus (ZIKV) infection in the human renal compartment has not been reported. Several clinical reports have describe high-level persistent viral shedding in the urine of infected patients, but the associated mechanisms have not been explored until now. The current study examined cellular components of the glomerulus of the human kidney for ZIKV infectivity. METHODS: I infected primary human podocytes, renal glomerular endothelial cells (GECs), and mesangial cells with ZIKV. Viral infectivity was analyzed by means of microscopy, immunofluorescence, real-time reverse-transcription polymerase chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines interleukin 1?, interferon ?, and RANTES (regulated on activation of normal T cells expressed and secreted) were assessed using qRT-PCR. RESULTS: I show that glomerular podocytes, renal GECs, and mesangial cells are permissive for ZIKV infection. ZIKV infectivity was confirmed in all 3 cell types by means of immunofluorescence staining, RT-PCR, and qRT-PCR, and qRT-PCR analysis revealed increased transcriptional induction of interleukin 1?, interferon ?, and RANTES in ZIKV-infected podocytes at 72 hours, compared with renal GECs and mesangial cells. CONCLUSIONS: The findings of this study support the notion that the glomerulus may serve as an amplification reservoir for ZIKV in the renal compartment. The impact of ZIKV infection in the human renal compartment is unknown and will require further study. Twit Text FOAVip Zika Virus Infection of the Human Glomerular Cells: Implications for Viral Reservoirs and Renal Pathogenesis ????J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=28398522 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28398522 #FOAvip English Wikipedia <ref>{{Cite pmid|28398522 }}</ref> Zika Virus Infection of the Human Glomerular Cells: Implications for Viral Reservoirs and Renal Pathogenesis #MMPMID28398522 Alcendor DJ J Infect Dis 2017[Jul]; 216 (2 ): 162-171 PMID28398522 show ga BACKGROUND: Zika virus (ZIKV) infection in the human renal compartment has not been reported. Several clinical reports have describe high-level persistent viral shedding in the urine of infected patients, but the associated mechanisms have not been explored until now. The current study examined cellular components of the glomerulus of the human kidney for ZIKV infectivity. METHODS: I infected primary human podocytes, renal glomerular endothelial cells (GECs), and mesangial cells with ZIKV. Viral infectivity was analyzed by means of microscopy, immunofluorescence, real-time reverse-transcription polymerase chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines interleukin 1?, interferon ?, and RANTES (regulated on activation of normal T cells expressed and secreted) were assessed using qRT-PCR. RESULTS: I show that glomerular podocytes, renal GECs, and mesangial cells are permissive for ZIKV infection. ZIKV infectivity was confirmed in all 3 cell types by means of immunofluorescence staining, RT-PCR, and qRT-PCR, and qRT-PCR analysis revealed increased transcriptional induction of interleukin 1?, interferon ?, and RANTES in ZIKV-infected podocytes at 72 hours, compared with renal GECs and mesangial cells. CONCLUSIONS: The findings of this study support the notion that the glomerulus may serve as an amplification reservoir for ZIKV in the renal compartment. The impact of ZIKV infection in the human renal compartment is unknown and will require further study. |Cells, Cultured [MESH] |Cytokines/immunology [MESH] |Fluorescent Antibody Technique [MESH] |Humans [MESH] |Kidney Glomerulus/cytology/pathology/*virology [MESH] |Mesangial Cells/*virology [MESH] |Podocytes/*virology [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Zika Virus Infection/*immunology/pathology [MESH]Zika Virus Infection of the Human Glomerular Cells: Implications for V(epmc).pdf DeepDyve Pubget Overpricing