Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28645173
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Spatially resolved metabolic analysis reveals a central role for transcriptional
control in carbon allocation to wood
#MMPMID28645173
Roach M
; Arrivault S
; Mahboubi A
; Krohn N
; Sulpice R
; Stitt M
; Niittylä T
J Exp Bot
2017[Jun]; 68
(13
): 3529-3539
PMID28645173
show ga
The contribution of transcriptional and post-transcriptional regulation to
modifying carbon allocation to developing wood of trees is not well defined. To
clarify the role of transcriptional regulation, the enzyme activity patterns of
eight central primary metabolism enzymes across phloem, cambium, and developing
wood of aspen (Populus tremula L.) were compared with transcript levels obtained
by RNA sequencing of sequential stem sections from the same trees. Enzymes were
selected on the basis of their importance in sugar metabolism and in linking
primary metabolism to lignin biosynthesis. Existing enzyme assays were adapted to
allow measurements from ~1 mm3 sections of dissected stem tissue. These
experiments provided high spatial resolution of enzyme activity changes across
different stages of wood development, and identified the gene transcripts
probably responsible for these changes. In most cases, there was a clear positive
relationship between transcripts and enzyme activity. During secondary cell wall
formation, the increases in transcript levels and enzyme activities also matched
with increased levels of glucose, fructose, hexose phosphates, and UDP-glucose,
emphasizing an important role for transcriptional regulation in carbon allocation
to developing aspen wood. These observations corroborate the efforts to increase
carbon allocation to wood by engineering gene regulatory networks.
free
free
free
