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Single-cell RNA-seq analysis unveils a prevalent epithelial/mesenchymal hybrid
state during mouse organogenesis
#MMPMID29540203
Dong J
; Hu Y
; Fan X
; Wu X
; Mao Y
; Hu B
; Guo H
; Wen L
; Tang F
Genome Biol
2018[Mar]; 19
(1
): 31
PMID29540203
show ga
BACKGROUND: Organogenesis is crucial for proper organ formation during mammalian
embryonic development. However, the similarities and shared features between
different organs and the cellular heterogeneity during this process at
single-cell resolution remain elusive. RESULTS: We perform single-cell RNA
sequencing analysis of 1916 individual cells from eight organs and tissues of
E9.5 to E11.5 mouse embryos, namely, the forebrain, hindbrain, skin, heart,
somite, lung, liver, and intestine. Based on the regulatory activities rather
than the expression patterns, all cells analyzed can be well classified into four
major groups with epithelial, mesodermal, hematopoietic, and neuronal identities.
For different organs within the same group, the similarities and differences of
their features and developmental paths are revealed and reconstructed.
CONCLUSIONS: We identify mutual interactions between epithelial and mesenchymal
cells and detect epithelial cells with prevalent mesenchymal features during
organogenesis, which are similar to the features of intermediate
epithelial/mesenchymal cells during tumorigenesis. The comprehensive
transcriptome at single-cell resolution profiled in our study paves the way for
future mechanistic studies of the gene-regulatory networks governing mammalian
organogenesis.
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