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Liang Y
; Chen S
; Yang Y
; Lan C
; Zhang G
; Ji Z
; Lin H
J Biomed Sci
2018[Mar]; 25
(1
): 25
PMID29540226
show ga
BACKGROUND: To investigate the treatment effect of vasoactive intestinal peptide
(VIP) on osteoarthritis (OA) and the relative mechanism. METHOD: The OA model on
the SD rat knee was established using the modified Hulth method, and the
recombinant pcDNA3.1+/VIP plasmid was constructed. One month after the plasmids
VIP were injected intra-articularly into the right knee joint of OA and
sham-operated rats, the pathological changes of the OA knee joint were observed
by Hematoxylin-eosin (HE) and Safranin O/fast green staining. The levels of VIP
and serum inflammatory cytokines (TNF-?, IL-2 and IL-4) were measured by ELISA
kits. Meanwhile, synoviocytes isolated from OA rat and sham-operated rat were
cultured in vitro, and transfected with the VIP plasmid. The proliferation of
synoviocytes was determined using BrdU kits. The protein expressions of TNF-?,
IL-2, CollagenII, osteoprotegerin (OPG), matrix-degrading enzymes (MMP-13,
ADAMTS-5), and the related protein of NF-?B signaling pathway (phosphorylated
p65, phosphorylated I?B?) were evaluated by western blot. RESULTS: The VIP
plasmid could effectively improve the pathological state of the OA rats knee
joint, significantly decrease the levels of serum TNF-? and IL-2, and clearly
increase the levels of VIP and serum IL-4. At the same time, after the OA
synoviocytes were treated with the VIP plasmid, the proliferation ability of OA
synoviocytes was reduced, the protein expressions of Collagen II and OPG were
remarkably up-regulated, and the protein expressions of TNF-?, IL-2, MMP-13 and
ADAMTS-5 were significantly down-regulated. In addition, the p-p65 expression
decreased and p-I?B? expression increased. CONCLUSION: Osteoarthritis was
effectively treated by VIP via inhibiting the NF-?B signaling pathway.
|*Signal Transduction
[MESH]
|Animals
[MESH]
|Male
[MESH]
|NF-kappa B/*genetics/metabolism
[MESH]
|Osteoarthritis, Knee/*drug therapy
[MESH]
|Rats
[MESH]
|Rats, Sprague-Dawley
[MESH]
|Recombinant Proteins/genetics/therapeutic use
[MESH]
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