Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1186/s12964-018-0223-4 http://scihub22266oqcxt.onion/10.1186/s12964-018-0223-4 C5850916!5850916 !29534718     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29534718 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Commun+Signal 2018 ; 16 (1 ): 10 Nephropedia Template TP {{tp|p=29534718 |t=2018. Ras enhances TGF-? signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1 |pdf=|usr=}}{{29534718 }} gab.com Text Ras enhances TGF- signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1 JO: Cell Commun Signal http://www.kidney.de/pget.php?&tw=1&pmid=29534718 #FOAvip BACKGROUND: Transformation by oncogene Ras overcomes TGF-? mediated growth inhibition in epithelial cells. However, it cooperates with each other to mediate epithelial to mesenchymal transition (EMT). The mechanism of how these two pathways interact with each other is controversial. METHODS: Molecular techniques were used to engineer expression plasmids for Ras, SPRY, TGF-? receptors, type I and II and ubiquitin. Immunoprecipitation and western blots were employed to determine protein-protein interactions, preotein levels, protein phosphorylation while immunofluorecesent staining for molecular co-localization. TGF-? signalling activities is also determined by its luciferase reporter assay. Trans-well assays were used to measure cell migration and invasion. RESULTS: Ras interacts with the SPSB1's SPRY domain to enhance TGF-? signaling. Ras interacts and colocalizes with the TGF-? type II receptor's (T?RII) negative regulator SPSB1 on the cell membrane, consequently promoting SPSB1 protein degradation via enhanced mono- and di-ubiquitination. Reduced SPSB1 levels result in the stablization of T?RII, in turn the increase of receptor levels significantly enhance Smad2/3 phosphorylation and signaling. Importantly, forced expression of SPSB1 in Ras transformed cells suppresses TGF-? signaling and its mediated migration and invasion. CONCLUSION: Ras positively cooperates with TGF-? signaling by reducing the cellular protein levels of T?RII negative regualtor SPSB1. Twit Text FOAVip Ras enhances TGF- signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1 ????Cell Commun Signal http://www.kidney.de/pget.php?&tw=1&pmid=29534718 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29534718 #FOAvip English Wikipedia <ref>{{Cite pmid|29534718 }}</ref> Ras enhances TGF-? signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1 #MMPMID29534718 Liu S ; Iaria J ; Simpson RJ ; Zhu HJ Cell Commun Signal 2018[Mar]; 16 (1 ): 10 PMID29534718 show ga BACKGROUND: Transformation by oncogene Ras overcomes TGF-? mediated growth inhibition in epithelial cells. However, it cooperates with each other to mediate epithelial to mesenchymal transition (EMT). The mechanism of how these two pathways interact with each other is controversial. METHODS: Molecular techniques were used to engineer expression plasmids for Ras, SPRY, TGF-? receptors, type I and II and ubiquitin. Immunoprecipitation and western blots were employed to determine protein-protein interactions, preotein levels, protein phosphorylation while immunofluorecesent staining for molecular co-localization. TGF-? signalling activities is also determined by its luciferase reporter assay. Trans-well assays were used to measure cell migration and invasion. RESULTS: Ras interacts with the SPSB1's SPRY domain to enhance TGF-? signaling. Ras interacts and colocalizes with the TGF-? type II receptor's (T?RII) negative regulator SPSB1 on the cell membrane, consequently promoting SPSB1 protein degradation via enhanced mono- and di-ubiquitination. Reduced SPSB1 levels result in the stablization of T?RII, in turn the increase of receptor levels significantly enhance Smad2/3 phosphorylation and signaling. Importantly, forced expression of SPSB1 in Ras transformed cells suppresses TGF-? signaling and its mediated migration and invasion. CONCLUSION: Ras positively cooperates with TGF-? signaling by reducing the cellular protein levels of T?RII negative regualtor SPSB1. |Animals [MESH] |B30.2-SPRY Domain [MESH] |Cell Movement/drug effects [MESH] |Cycloheximide/pharmacology [MESH] |Dogs [MESH] |Down-Regulation/drug effects [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Leupeptins/pharmacology [MESH] |Madin Darby Canine Kidney Cells [MESH] |Mutagenesis, Site-Directed [MESH] |Phosphorylation/drug effects [MESH] |Protein Binding [MESH] |Receptor, Transforming Growth Factor-beta Type II/metabolism [MESH] |Signal Transduction/drug effects [MESH] |Smad2 Protein/metabolism [MESH] |Suppressor of Cytokine Signaling Proteins/chemistry/genetics/*metabolism [MESH] |Transforming Growth Factor beta/genetics/*metabolism/pharmacology [MESH] |Ubiquitination [MESH]Ras enhances TGF-? signaling by decreasing cellular protein levels of (epmc).pdf DeepDyve Pubget Overpricing