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10.1093/rheumatology/kex088 http://scihub22266oqcxt.onion/10.1093/rheumatology/kex088 C5850724!5850724!28398552     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28398552&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Rheumatology+(Oxford) 2017 ; 56 (7): 1217-26 Nephropedia Template TP {{tp|p=28398552|t=2017. Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies |pdf=|usr=}}{{28398552}} gab.com Text Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies JO: Rheumatology (Oxford) http://www.kidney.de/pget.php?&tw=1&pmid=28398552 #FOAvip Objectives. Anti-carbamylated protein (anti-CarP) antibodies are detected in RA patients. Fetal calf serum is used as an antigen source in anti-CarP ELISA, and the precise target antigens have not been found. We aimed to identify the target antigens of anti-CarP antibodies.Methods. Western blotting of anti-CarP antibodies was conducted. Anti-carbamylated human albumin (CarALB) antibody was detected by in-house ELISA for 493 RA patients and 144 healthy controls (HCs). An inhibition ELISA of anti-CarP antibodies by CarALB and citrullinated albumin (citALB) was performed using eight RA patients? sera. Serum CarALB was detected by liquid chromatography?tandem mass spectroscopy (LC/MS/MS), and the serum MPO concentration was measured by ELISA.Results. We focused on carbamylated albumin because it corresponded to the size of the thickest band detected by western blotting of anti-CarP antibodies. Anti-CarALB antibody was detected in 31.4% of RA patients, and the correlation of the titres between anti-CarALB and anti-CarP was much closer than that between anti-citALB and anti-CCP antibodies (? = 0.59 and ? = 0.16, respectively). The inhibition ELISA showed that anti-CarP antibodies were inhibited by CarALB, but not by citALB. CarALB was detected in sera from RA patients by LC/MS/MS. The serum MPO concentration was correlated with disease activity and was higher in RA patients with anti-CarALB antibody than in those without.Conclusion. We found that carbamylated albumin is a novel target antigen of anti-CarP antibodies, and it is the first reported target antigen that has not been reported as the target of ACPA. Twit Text FOAVip Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies ????Rheumatology (Oxford) http://www.kidney.de/pget.php?&tw=1&pmid=28398552 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28398552 #FOAvip English Wikipedia <ref>{{Cite pmid|28398552}}</ref> Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies #MMPMID28398552 Nakabo S; Hashimoto M; Ito S; Furu M; Ito H; Fujii T; Yoshifuji H; Imura Y; Nakashima R; Murakami K; Kuramoto N; Tanaka M; Satoh J; Ishigami A; Morita S; Mimori T; Ohmura K Rheumatology (Oxford) 2017[Jul]; 56 (7): 1217-26 PMID28398552show ga Objectives. Anti-carbamylated protein (anti-CarP) antibodies are detected in RA patients. Fetal calf serum is used as an antigen source in anti-CarP ELISA, and the precise target antigens have not been found. We aimed to identify the target antigens of anti-CarP antibodies.Methods. Western blotting of anti-CarP antibodies was conducted. Anti-carbamylated human albumin (CarALB) antibody was detected by in-house ELISA for 493 RA patients and 144 healthy controls (HCs). An inhibition ELISA of anti-CarP antibodies by CarALB and citrullinated albumin (citALB) was performed using eight RA patients? sera. Serum CarALB was detected by liquid chromatography?tandem mass spectroscopy (LC/MS/MS), and the serum MPO concentration was measured by ELISA.Results. We focused on carbamylated albumin because it corresponded to the size of the thickest band detected by western blotting of anti-CarP antibodies. Anti-CarALB antibody was detected in 31.4% of RA patients, and the correlation of the titres between anti-CarALB and anti-CarP was much closer than that between anti-citALB and anti-CCP antibodies (? = 0.59 and ? = 0.16, respectively). The inhibition ELISA showed that anti-CarP antibodies were inhibited by CarALB, but not by citALB. CarALB was detected in sera from RA patients by LC/MS/MS. The serum MPO concentration was correlated with disease activity and was higher in RA patients with anti-CarALB antibody than in those without.Conclusion. We found that carbamylated albumin is a novel target antigen of anti-CarP antibodies, and it is the first reported target antigen that has not been reported as the target of ACPA. äCarbamylated albumin is one of the target antigens of anti-carbamylate(epmc).pdf DeepDyve Pubget Overpricing