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10.1021/acsnano.6b07600 http://scihub22266oqcxt.onion/10.1021/acsnano.6b07600 C5848212!5848212!28129503     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Nano 2017 ; 11 (3): 2452-8 Nephropedia Template TP {{tp|p=28129503|t=2017. Efficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein |pdf=|usr=}}{{28129503}} gab.com Text Efficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein JO: ACS Nano http://www.kidney.de/pget.php?&tw=1&pmid=28129503 #FOAvip Genome editing through the delivery of CRISPR/Cas9-ribonulceoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here we report a remarkably high efficient (~90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the co-engineering of Cas9 protein and carrier nanoparticles. This construct provides effective (~30%) gene editing efficiency and opens up new opportunities in studying genome dynamics. Twit Text FOAVip Efficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein ????ACS Nano http://www.kidney.de/pget.php?&tw=1&pmid=28129503 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28129503 #FOAvip English Wikipedia <ref>{{Cite pmid|28129503}}</ref> Efficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein #MMPMID28129503 Mout R; Ray M; Tonga GY; Lee YW; Tay T; Sasaki K; Rotello VM ACS Nano 2017[Mar]; 11 (3): 2452-8 PMID28129503show ga Genome editing through the delivery of CRISPR/Cas9-ribonulceoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here we report a remarkably high efficient (~90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the co-engineering of Cas9 protein and carrier nanoparticles. This construct provides effective (~30%) gene editing efficiency and opens up new opportunities in studying genome dynamics. äEfficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas(epmc).pdf DeepDyve Pubget Overpricing