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2013 ; 88
(6
): 749-57
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Detection of two biological markers of intercourse: prostate-specific antigen and
Y-chromosomal DNA
#MMPMID24028752
Jamshidi R
; Penman-Aguilar A
; Wiener J
; Gallo MF
; Zenilman JM
; Melendez JH
; Snead M
; Black CM
; Jamieson DJ
; Macaluso M
Contraception
2013[Dec]; 88
(6
): 749-57
PMID24028752
show ga
BACKGROUND: Although biological markers of women's exposure to semen from vaginal
intercourse have been developed as surrogates for risk of infection or
probability of pregnancy, data on their persistence time and clearance are
limited. STUDY DESIGN: During 2006-2008, 52 couples were enrolled for three
14-day cycles of abstinence from vaginal sex during which women were exposed in
the clinic to a specific quantity (10, 100 or 1000 ?L) of their partner's semen.
Vaginal swabs were collected before and at 1, 6, 12, 24, 48, 72 and 144 h after
exposure for testing for prostate-specific antigen (PSA) and Y-chromosome DNA (Yc
DNA). RESULTS: Immediately after exposure to 1000 ?L of semen, the predicted
sensitivity of being PSA positive was 0.96; this decreased to 0.65, 0.44, 0.21
and 0.07 at 6, 12, 24 and 48 h, respectively. Corresponding predicted sensitivity
of being Yc DNA positive was 0.72 immediately postexposure; this increased to
0.76 at 1 h postexposure and then decreased to 0.60 (at 6 h), 0.63 (at 12 h),
0.49 (at 24 h), 0.21 (at 48 h), 0.17 (at 72 h) and 0.12 (at 144 h). CONCLUSIONS:
Overall findings suggest that PSA may be more consistent as a marker of very
recent exposure and that Yc DNA is more likely to be detected in the vagina after
12 h postexposure compared to PSA.