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2018 ; 13
(3
): e0193164
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English Wikipedia
Mosquito pornoscopy: Observation and interruption of Aedes aegypti copulation to
determine female polyandric event and mixed progeny
#MMPMID29518112
Carvalho DO
; Chuffi S
; Ioshino RS
; Marques ICS
; Fini R
; Costa MK
; Araújo HRC
; Costa-da-Silva AL
; Kojin BB
; Capurro ML
PLoS One
2018[]; 13
(3
): e0193164
PMID29518112
show ga
Ades aegypti is the most important arbovirus vector in the world, and new
strategies are under evaluation. Biological studies mentioning the occurrence of
a second mate in Aedes aegypti can interfere with vector control program
planning, which involves male mosquito release technique. This study presents
different experiments to show the occurrence of mixed progeny. Mixed male crosses
(using a combination of different type of males in confinement with virgin
females) showed no polyandric female. Individual crosses with male substitution
in every gonotrophic cycle also did not show any polyandric female. Individual
crosses with a 20 minutes interval, with subsequent male change, showed that only
a few females presented mixed offspring. The copulation breach in three different
moments, group A with full coitus length, group B the coitus was interrupted in
5-7 seconds after the start; and group C, which the copulation was interrupted 3
seconds after started. In summary, group A showed a majority of unique progeny
from the first male; group B showed the higher frequency of mixed offspring and
group C with the majority of the crosses belonging to the second male. To
conclude, the occurrence of a viable second mate and mixed offspring is only
possible when the copulation is interrupted; otherwise, the first mate is
responsible for mixed progeny.